The Resistance Spectrum Of Enterobacteriaceae, An Anal Culture Isolate
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Examining the Resistance Spectrum of Enterobacteriaceae, An Anal Culture Isolate
The method by which antibiotics aim to treat diseases caused by bacteria can be by efficiently preventing cell wall and protein synthesis or by disrupting DNA replication, resulting in either killing the pathogenic bacteria (cidal) or stalling their growth (static). The most prevalent method of resistance is the bacterium 's possession of plasmids exposing a CTX gene fragment resulting in expressing β-lactamase to hydrolyze β-lactams, making antibiotic targeting by cephalosporins highly inefficient (Wang et al., 2015). Unfortunately, the augmented use and abuse of antibiotics in agriculture, hospitals, and the community over the years has posed serious health…show more content… This disk diffusing technique is analyzed by measuring the zone of inhibition which is then compared to the interpretative standards depicted in the table illustrating performance for antimicrobial susceptibility testing. For instance, a bacterial strain showing Penicillin G resistance would produce a zone of inhibition of ≤ 26mm. The Kirby-Bauer technique is a simple and effective assessment since one is able to determine the resistance or susceptibility of a bacterial strain against several antibiotics at a time.
The goal of this experiment is on investigating the resistance of the bacterial strain isolated from the anal culture to twelve common antibiotics. The Gram-negative bacterial strain that was isolated from the anal culture was identified as being a facultative organism part of the Enterobacteriaceae family. It is hypothesized to be susceptible to Amoxicillin, Ciprofloxacin, and Piperacillin. Furthermore, according to the spectrum of activity on the interpretive standards table, Enterobacteriacea, being gram negative, would be susceptible to Cephalothin, Gentamicin, Polymyxin B, Sulfadiazine, and Tetracycline. The results from this assessment can be used to fuel the inquiry to investigate possible mechanisms and genetic mutations that Enterobacteriaceae exploits to seek resistance against antibiotics that are ineffective against this strain.
The purpose of the Kirby-Bauer test