In Biochemistry, Matthews 4th Edition the COX-2 is a bifunctional endoplasmic reticulum membrane enzyme with two activities in single heme-containing polypeptide chain. The first, a cyclooxygenase activity , introduces two molecules of O2 and gives PGG2. The cyclooxygenase reaction is one of the first steps in eicosanoid synthesis for prostaglandins. The second activity, a peroxidase, is a two electron reduction of the peroxide to give PGH2. PGH2 is a prostaglandin endoperoxide that serves as a precursor to other prostaglandins. COX-2 specifically is induced by cytokines, mitogens, and endotoxins in the inflammatory cells and is responsible for the elevated production of prostaglandins during inflammation. P53 is a tumor suppressor …show more content…
Heptocytes are the cells of the main tissue in the liver. The article describes the relationship between protein and lipid oxidation with the expression of COX-2 and p53 genes in adult MSCs during their trans-differentiation into hepatocyte-like cells. The most pivotal data within this article is the correlation of the p53 down-regulation with the expression of COX-2 during the differentiation of MSCs into hepatocytes. The changes in the COX-2 and p53 expression in relation to oxidative stress were studied using a quantitative polymerase chain reaction at mRNA levels over the course of 21 days with cell samples taken every 7 days. The authors isolated the mononuclear cells in a culture medium in a CO2 incubator. The non-adherent cells were removed by replacement of the culture every 3 days so that only the fibroblast-like adherent cells remained which gave the MSCs. To induce the hepatocyte differentiation of the MSCs they carried out a two-step protocol employing HGF, DEX, and oncostatin M to promote hepatocyte differentiation.
The first bar chart demonstrates the COX-2 expression at mRNA levels during the hepatocyte differentiation of MSCs. The second bar chart demonstrates p53 expression at mRNA levels in MSCs and hepatocytes cells during differentiation. All the assays were carried out in triplicate with cell samples taken at each time point. The authors
Hepatocytes are involved in synthesizing proteins, cholesterol, bile salts, fibrinogen, phospholipids and glycoproteins. Additionally, hepatocytes ensure that our
HeLa cells are the most widely used human cell lines in existence. They are used in many experiments. For example, recent studies have shown that expression of girdin is associated with types of cancer. The expression of gidin was suppressed when in HeLa cells in vitro which allowed the gidin to be evaluated. It was found that patients with high-grade cervical cancer tumors exhibited strong expression of girdin. It appeared that girdin is key in HeLa cell
Besides inducing apoptosis and controlling the cell cycle, p53 has been demonstrated to be a central component and key regulator of the metabolic stress machinery. The metabolic balance between glycolysis and oxidative phosphorylation is heavily coordinated by p53 activity, which is activated by
Promoting or inhibiting the transcription of genes that code for the synthesis of cellular proteins intracellular second-messenger system are correct
One study by Shuijun Zhanga et al had shown the ABT-737 induced apoptosis of hepatocellular carcinoma cells (HCC) by the transcriptional repression of Myeloid cell leukemia 1 (Mcl-1). Shuijun Zhanga et a. had shown that NCTD could affect apoptosis by the modification of the B-cell lymphoma 2
Half of the animals will be euthanized on day 28 (before the second DSS cycle), and the other half on day 56. This will allow us to study ROS production and DNA stability before the onset of neoplasia (28 days), and once it is established (56 days). We have an extensive experience in this animal model6,8,10. Power calculations indicate we will need an n=8 mice per group to obtain significant results31. Colon will be processed for histology and epithelial cell isolation by chelation in EDTA. Part of these epithelial cells will be kept for RNA isolation, part will be used for ROS determination, and the rest will be seeded in extracellular matrices to grow them as organoids. Organoids will be cultured for 7 days to allow for additional divisions in the presence or absence of 1µg/mL LPS32. Epithelial cells in tissues and culture will be analyzed for DNA damage and genetic instability via γH2AX immunofluorescence (a marker for DNA damage), presence of anaphase bridges, and telomere staining. Dr. Verdun´s lab is very experienced in these techniques33,34. Epithelial cells will be interrogated for the expression of Duox2, SOD1, CAT, GPX2, and GPX4 (quantitative PCR) as markers of oxidative stress in epithelial cells35, and production of ROS will be measured by staining with DCFDA and Amplex Red probes. These experiments will indicate to which extent DNA damage induced by ROS is mediated by TLR4 activation in the
The cells were washed with PBS and then incubated in serum-free media and treated with 2.5 μM of free sorafenib or an equivalent sorafenib concentration of SMA-Sor, 3 μM of free nilotinib or an equivalent nilotinib concentration of SMA-Nilo, DMSO or SMA for 48 h. Following treatment, media was collected, centrifuged to remove cell debris, and freeze-dried for 12 h. Samples were rehydrated and mixed with loading buffer (0.4 M Tris, pH 6.8, 5% SDS, 20% glycerol, 0.03% bromophenol blue). For zymography, samples were loaded on a 10% SDS-polyacrylamide gel containing 1 mg/mL of gelatin. After electrophoresis, the gels were incubated in renaturing solution (2.5% Triton-X-100 (w/v)) for 30 min at room temperature and then for 24 h at 37°C in a developing buffer containing 50 mM Tris, pH 7.5, 200 mM NaCl, 4 mM CaCl2, and 0.02% NP40. The gels were then stained with Coomassie blue R250, and regions without staining were indicative of gelatin lysis. The gels were briefly rinsed and scanned. For MMP-9 and isthmin-1 secretion, samples were loaded on a 10% SDS-polyacrylamide gel, and the expression of MMP-9 and isthmin-1 assessed by western blotting using anti-MMP-9 (D6O3H, Cell Signaling) and anti-isminth-1 antibodies (Biorbyt, San Francisco, CA,
Thus, there is an urgency to evaluate the effects of immune response on APAP toxicity using a human KCs and hepatocyte co-culture system. Since a 3D multicellular system containing hepatocytes and other non-hepatocytes can better reflect the cell-cell interactions of a real situation [11], in this study, we used the 3D liver microtissues consisting of primary human hepatocytes (PHH) and KCs (1 : 1) to study the inflammation-mediated hepatotoxicity after acetaminophen overdose.
Compare and contrast tumour suppressor genes and proto-oncogenes. Discuss an example of how recent advances in our understanding of these genes have led to the development of a novel therapy that is being used in the treatment of human cancer.
COX or cyclo- oxygenase is a type of enzyme that is involved in the formation of prostaglandins. Prostaglandins is a nuisance to our body because it can cause a bunch of things like, inflammation, high body temperature, and pain. This is where the Aspirin come into action; it inhibits COX to prevent the formation of prostaglandins.
It has been suggested that abnormalities in the activity or expression of HDACs can lead to an imbalance of HDAC relative to histone acetyltransferase activity resulting in diminished expression of regulatory genes, which control the cell cycle and apoptosis developing tumorigenesis (Licciardi, Ververis, Hiong & Karagiannis, 2013). In some cases, HDAC enzymes are abnormally recruited to gene promoters, which constitutively repress gene expression causing cancer. In addition, HDACs alter gene expression due to the posttranslational deacetylation modification of various nonhistone protein substrates, such as DNA-binding proteins, DNA-repair proteins, transcription factors, signal-transduction molecules, and chaperone proteins(Licciardi, Ververis, Hiong & Karagiannis,
Methionine (Met) restriction has become the focus of interest as dietary regimen since the incidence of diseases such as, cardiovascular disorders, diabetes and cancers (Anderson and Weindruch, 2012) are rising at a higher rate, posing health care challenges. Met is the first limiting AA in poultry diet. It has been used to compliment cancer treatment in humans (1) and improve metabolic health (2, 3). However, the liver also plays a vital role in Met metabolism giving that almost half of the daily intake is metabolized there. In addition to its action and as part of the one-carbon metabolic cycle, it can perform critical role in generating S-adenosylmethionine (SAM) which is the major methyl donor for a variety of biologically important reactions.
Considerable research has been done over the past few years focused mainly on identifying molecular events in pancreatic carcinogenesis, and the correlation with clinic pathological status (Sarkar, 2007). With that being said there has been many discoveries along the lines of symptom recognition to the point that like symptoms can be evaluated simplified. Within all that it was discovered that there were multiple subsets of genes that undergo changes genetically. The activation of oncogenes and the inactivation of tumor suppressor genes are partly
In this connection, the role of the macrophage has already been highlighted. An additional factor limiting the regression of established fibrosis is the previously mentioned increased survival of activated myofibroblasts. Increased expression of anti-apoptotic pathways is a hallmark of chronic myofibroblast activation and, for example, expression of the anti-apoptotic protein B-cell lymphoma gene-2 (Bcl-2) is markedly evident in myofibroblast-like cells that are present in areas of fibrosis in liver tissue obtained from patients with HCV-related cirrhosis. It is, therefore, plausible that long-term fibrogenesis is characterized, in addition to the biochemical evolution of scar tissue and the lack of appropriate degradation machinery, by the immovability of a critical mass of profibrogenic cells (Iredale,
Accumulation of lipid intermediates in hepatocytes causes hepatocellular lipotoxicity, leading to cellular stress, dysfunction and eventually cell death. Lipotoxicity-induced hepatocyte cell death appears to be mainly mediated by the apoptotic machinery activated by death receptors and endoplasmic reticulum (ER) stress.5 6 Other cell death modes, such as necroptosis and pyroptosis, have also been reviewed recently.6 7 However, the contribution of necroptosis in lipid-induced hepatocyte death during NASH needs further definition.6 8 Albeit growing evidence supports a role for the inflammasome in NASH development, the contribution of hepatocyte pyroptosis to hepatocellular injury and death also needs to be