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Therapeutic Patterns In Genetic Analysis

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The first thing that I would go through is the method to identify the QTL. Using molecular markers to identify the presence or absence at certain lengths of DNA coupled with observed phenotypic data. In order to obtain a QTL, markers methods described above are used in order to get information from different locations within the target organisms chromosome. The organism in question has a DNA sample taken from it. At the same time, the associated trait that you are interested in (such as disease resistance) is tested for in the organism. Because of the differences between organisms and the divergence of traits, a population is generally used in order to help determine the presence of the QTL. Generally, a cross between a resistant and a susceptible individual for the trait is done, and the population that is …show more content…

The LOD score is a measure of how much the trait is depicted by a certain marker. Having one or a handful of locations that make up for the trait in question is important because it allows you to have a better idea and a simplified method for determining whether the trait of interest is present. The location is also vital, as it allows you to know where the trait of interest is located at. This is important for two reasons. If you are trying to sequence the trait to get an idea of the protein structure, this gives you the ability to know the base pair sequence, so you know what amino acids are coded and have an idea of the protein structure. For a QTL cloning project, however, the location of the QTL or QTL's is important because it lets you use molecular cloning techniques in order to copy the QTL from one organism to another. Once you have the QTL's location, cloning techniques can be utilized in order to transfer the QTL of interest. The techniques that I will talk about are gene cloning using a vector, polymerase chain reaction, and gene

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