Western Blot Analysis

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The technique used in this experiment was western blot to determine the protein levels in different cow’s stages; fetal calf serum, newborn calf serum and cow serum. Western blot is technique commonly used to identify proteins by its movement in the gel electrophoresis. Western blot is use to separate protein based on its molecular weight in gel electrophoresis. The proteins separated in the gel, then transferred to a nitrocellulose membrane using an electron current (1). Finally the membrane is incubated with proteins that would stain with antibodies specific for the wanted protein. SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is used in this experiment to detect the presence of antibodies in different serum samples
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