determine the activity of G6Pase Essay

To make the results of the experiment valid four variables to take into account are if the freezer is the same temperature for both tests, the water is the same water just different temperatures, the ice cube trays are the same size, and finally both trays are in the freezer for the same amount of time.

The dependent variable in the experiment was the temperature and energy absorbed by the water.

Because of this, many possible sources of error in the experiment have to do with water temperature changing. One possible source of error was that the water surrounding the Daphnia magna was constantly returning to room temperature. Once the 30° C water was taken off the hot plate and put in the dish, it was always cooling down to reach room temperature, 21° C, by losing heat to the air. So that the heart rate found during the three different trials may have been at varying temperatures, some cooler than 30° C. Once the 10° C water was put in the dish, it was always warming up to reach room temperature, by gaining heat from the air. This may have caused the heart rate that was found during the three different trials to have been at varying temperatures, some warmer than 10°

In the lab the total volume of solutions used is kept constant in all the trials so that the temperature change remains directly proportional to the amount of reaction that occurs as well to the extent of the reaction.

Controls- The control in this experiment was very important because if it was not contained, then the data would have been faulty. It was very difficult to keep

    One possible source of error that can affect the results was that a mercury thermometer was used instead of an electronic one. The use of a mercury

One of our flaws was that the temperature of the water was not exactly the same when we did the different trials. The temperatures were slightly off from our recorded value during the experiments. The change in temperature would affect the time it took for the Alka-Seltzer tablet to dissolve in the water. If there was a direct relationship between water temperature and dissolve time, we would not be able to see it because the temperatures are off and the dissolve times are not associated with the correct temperature. Another flaw is that we did not use the same amount of water throughout the experiment. We used a beaker to measure the water, which did not result in accurate measurements. The difference in amount of water could result in a difference in reaction time. The third flaw in the experiment was that during the reaction of the warm water, the water in the cup overflowed and spilled, bringing some of the Alka-Seltzer tablet with it. There were different amounts of tablet in different areas of the water, which means a different amount of Alka-Seltzer remained inside of the cup in each trial. This difference would mean that data for the warm water would fluctuate and we would not have accurate

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

The experiment is repeated twice and an average is obtained. This ensures the consistency of the measurement as the reading is triplicated, thus reducing anomalies. It can be seen that the reading obtained were around the same value. This proves that the results are reliable.

What other conditions that may affect the action of enzymes? Substrates could affect the action of the

The difference was in how long it took for the bubbles to come to the surface and the solution to become clear. Also, when doing the lab, once the correct temperature for the water was reached, the experimenter pulled the beakers out one by one completing all 5 trials. This may have caused slight fluctuations in data because by the time the 4 trials were finished, the temperature of the beaker may have increased by a few degrees. As for the act of measuring, there was a slight error because the experimenters did not take the beakers off the hot plate at exactly 30.00 degrees, or whatever the temperature may be. In order to provide more accurate results, the experimenter could do all

After the substrate solution was added, five drops of the enzyme were quickly placed in tubes 3, 4 and 5. There were no drops of enzyme added in tubes 1 and 2 and in tube 6 ten drops were added. Once the enzyme solution has been added the tubes were then left to incubate for ten minutes and after five drops of DNSA solution were added to tubes 1 to 6. The tubes were then placed in a hot block at 80-90oC for five minutes. They were then taken out after the five minute period and using a 5 ml pipette, 5 ml of distilled water were added to the 6 tubes and mixed by inversion. Once everything was complete the 6 tubes were then taken to the Milton Roy Company Spectronic 21 and the absorbance of each tube was tested.

The substrates of glycerate kinase are ATP and D-glycerate and the products are ADP and 3-phospho-D-glycerate. This enzyme belongs to the family of transferases.1 Other common names include: glycerate kinase (phosphorylating), D-glycerate 3-kinase, D-glycerate kinase, glycerate-3-kinase, GK, D-glyceric acid kinase, and ATP: D-glycerate 2-phosphotransferase. 1 This enzyme participates in 3 metabolic pathways: serine/glycine/threonine metabolism, glycerolipid metabolism, and glyoxylate-dicarboxylate metabolism.1

This lab is performed in order to determine the total energy in a reaction between zinc and hydrochloric acid. The reaction is done twice, once to measure the heat of the reaction and again to determine the work done in the system. This is because Enthalpy equals heat plus work (∆H= ∆E+W). Heat and work can be broken down further into separate components so the equation used in lab is ∆H=mc∆T + PV. Many calculations are used in the lab to find out what cannot be measured directly (ex: volume). After all the calculations were complete it was shown to have a very small percent error.

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After