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Glycerate Rationale Lab Report

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Introduction
The substrates of glycerate kinase are ATP and D-glycerate and the products are ADP and 3-phospho-D-glycerate. This enzyme belongs to the family of transferases.1 Other common names include: glycerate kinase (phosphorylating), D-glycerate 3-kinase, D-glycerate kinase, glycerate-3-kinase, GK, D-glyceric acid kinase, and ATP: D-glycerate 2-phosphotransferase. 1 This enzyme participates in 3 metabolic pathways: serine/glycine/threonine metabolism, glycerolipid metabolism, and glyoxylate-dicarboxylate metabolism.1

Glycerate kinase is active in photosynthesis.2 Photosynthesis converts CO2 to useable energy.2 There are two different types of photosynthesis: light dependent, which harvests energy from sunlight and the electron transport chain to make ATP and NADPH.2 The second type is light independent, which does not require sunlight; it uses the Calvin Cycle to convert CO2 to ATP and NADPH.2

Glycerate kinase is also an intermediate in glycolysis. Class II glycerate kinase’s form glycerate 2-phosphate (2PGA).3 2PGA is an intermediate of glycolysis as it is a substrate for phosphoglycerate mutase and enolase.4 Phosphoglycerate mutase converts 3-phospho-D-glycerate (3PGA) to 2PGA and enolase converts phosphoenolypyruvate (PEP) to 2PGA.4 Photorespiratory Regulation …show more content…

The presence of atmospheric oxygen triggers the oxygenation of RubP, leading to the synthesis of equimolar amounts of 3PGA and 2PG. The ratio of 3PGA to 3PG synthesis is determined by both intrinsic properties of Rubisco and by the CO2:O2 ratio in the enzymes microenvironment. The photorespiratory C2 cycle acts as an ancillary metabolic pathway that compensates for the futile withdraw of RubP from the C3 cycle under aerobic conditions by serving as a carbon recovery system reconverting 2PG to

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