lab report 5 Essay

The protein molecules in many foods provide the amino acid building blocks required by our own cells to produce new proteins. To determine whether a sample contains protein, a reagent called Biuret solution is used. Biuret solution contains copper ions. However, the chemical state of the copper ions in Biuret solution causes them to form a chemical complex with the peptide bonds between amino acids (when present), changing the color of the solution. Biuret solution is normally blue, but changes to pink when short peptides are present and to violet when long polypeptides are present.

You can store them on the domain itself and I would say that this is the same as a PKI server because a PKI server has more of a hierarchal ladder of trust to verify users.

The purpose of the lab was to determine the ratio of air to acetylene results in complete combustion of acetylene gas. The balanced chemical equation for this experiment was C2H2(g) + O2(g) --> CO2(g)+ H2O(l). Complete combustion is the reaction of an element or compound with oxygen to produce the most common oxides and energy. Complete combustion occurs when the fuel and oxygen combine in exact proportions to completely burn the fuel, which leaves a clean test tube. Incomplete combustion is the reaction of an element or compound with oxygen to produce some oxides with less oxygen than the most common oxides. Incomplete combustion occurs when there is not enough oxygen to react

In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or

3. Experiment: Use the Gizmo to find the carrying capacity with Ample, Moderate, and Little land. List the carrying capacities below.

What observations can you make regarding the gene pool and gene frequency of the surviving individuals?

a. Change the 24th bead to a different color. What does the sentence say now (re-read the entire sentence)? Does the sentence still make sense?

groups. It was conducted to further the research in choice overload and paralysis of the mind.

this means that is the optimal temperature, but in bacterial occur in less time than in fungal

The purpose of the “chi-square test” was to see if our data was in an acceptable range of a specific ratio listed above. The chi-square test took into account the expected deviations in the F2 offspring’s alleles.

The environment determines which traits are most evolutionary desirable to an organism. If a population should move or should the environment change, a different set of traits would be the most advantageous

Source #1 and source #2 are all about how organisms (plants and animals) survive in their for an example in source #1 it says an ice fish can live in its environment which is Antarctica because they have something in their blood so crystals of ice can’t be formed in its body. source #1 also says that penguins snuggle together to keep themselves warm in their arctic environment. last a crab's environment can help it because some crabs live towards the bottom of the see but there are warm volcanos down there

An aliquot (5 mL or 500 ml) of sample was filtered through a Whatman Anotop PTFE syringe filter (0.2 µm) and the concentrations of target PHCs were quantified using HPLC-MS/MS. Question: Why not preconcentration such as passing 200 ml through a cartridge to improve sensitivity? How does this method compare with published SPE techniques?

The experiment gave us an insight of how pH affects catalase activity. We hypothesized that the enzyme activity will be quicker near neutral pH levels, than in acidic or basic solutions. The expectation was the optimum rate of enzyme would be at near or at neutral solution, while the rate would be really slow in acidic or basic solutions, such as in solutions of HCl and NaOH. Our experimentation ran into some errors because we used tap water which didn’t have a neutral pH of 7; instead it was 5, which is considered to be acidic. So, our experiment included pH’s of 0, 5, and 13.

In the article, the Worthington Biochemistry Corporation concisely explain the effects of pH and show what the optimal pH level is for multiple enzymes. The author/s are believed to be using data gained through experiments conducted, to try and identify the region of pH optimal stability for each enzyme. The research focuses on basic concepts of enzymology and how other factors can affect the enzymatic reaction of pH. This article is very helpful for researchers who must use pH buffer solutions but have little background knowledge on the effects pH has on enzymes such as catalase. The main limitation here is that the main topic isn’t explained in depth, thus the author/s indicate that in order to further develop a more in-depth understanding of the effects of pH, more extensive research needs to be undertaken. Although, this article will not form the basis of my research, it will however be useful supplementary information for my research on the effects of pH as it is an up-to-date article.