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1. concept map for antibody screening and identifications
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- Describe the principles behind direct and indirect fl uorescent antibody tests.Choose all that are correct about indirect ELISA. A) An antigen can bind the substrate B) A secondary antibody can bind the antigen C) A secondary antibody can bind a primary antibody D) A primary antibody can bind an antigen1. True or False: Antibodies for Western blotting can be applied directly to the SDS-PAGE gel.
- Describe 1 aspect that lead to antibody diversityAn MLS has completed an antibody panel using PEG. She cannot exclude anti-Fya, or anti-E. Anti-Kpª and anti-Ja cannot be excluded either but they are low incidence antigens and the MLS is not concerned with those at this time. What would be the next logical step to aid in antibody identification? a. Run the panel again using ficin treated cells b. Run the panel again using 22% albumin for enhancement c. Pay special attention to the strength of the agglutination reactions d. Run the panel again but increase the 37 degree incubation time to one hourWhat secondary antibody would I need if I purchase a rabbit anti Bax antibody?
- 1.Explain the mechanism as to how the following can be used to visualize cells: Antibodies and Fluorescent dyes. Then aside from hematoxylin, what dyes are commonly used in preparing histological slides? List and indicate when the dyes can be used, or on what samples can the dyes be used.Using flow diagrams show the main steps in antigen and antibody detection ELISA's.What are the important considerations that you have to remember in antibody screening? What are the important considerations that you have to remember in antibody identification What is the role of antibody screening in pretransfusion compatibility testing?
- True or false 1. Heterogenous antibodies used for structural studies can be obtained by monoclonal hybridoma technique. 2. PrP(Sc) is largely alpha-helical structure.Describe 2 methods of immunotherapy currently in use, and the clinical results of one of these (ie, how often does it work well?)What is an introduction to ANTIBODY STRUCTURE AND FUNCTION ? How would you summarize this to someone who is new to this? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726606/