Questions 14-16 are based on the following. In the 1940's, Avery, Macleod, and McCarty transformed nonencapsulated bacteria into encapsulated. forms by growing the nonencapsulated cells in a culture containing an extract made from dead encapsulated cells. The transformed cells produced colonies of encapsulated bacteria. Three different procedures and their results are outlined below. Procedure I: Extract made from dead encapsulated cells added to culture medium. Nonencapsulated bacteria added to culture medium. Results: Both nonencapsulated and encapsulated bacteria grow. Procedure II: Extract made from dead encapsulated cells treated with protein-degrading enzymes before adding extract to culture medium. Nonencapsulated bacteria added to culture medium. Results: Both nonencapsulated and encapsulated bacteria grow. Procedure III: Extract made from dead encapsulated cells treated with DNAse (an enzyme that selectively destroys DNA) before adding extract to culture medium. Nonencapsulated bacteria added to culture medium. Results: Only nonencapsulated bacteria grow. 14. A reasonable conclusion to draw from the results of the experiment is that (A) DNA is the genetic material (B) DNA replication is semiconservative (C) DNA is a double helix (D) DNA is translated into protein (E) mutation is a change in the genetic material 15. What was the purpose of treating the extract with protein-degrading enzymes in Procedure II? (A) To demonstrate that the transforming factor is an enzyme (B) To demonstrate that the transforming factor is not a protein (C) To destroy nucleic acids in the extract (D) To destroy any capsules in the extract (E) To prevent the extract from being contaminated by nonencapsulated bacteria 16. What was the purpose of treating the extract with DNAse in Procedure III? (A) To remove the encapsulated bacteria from the extract (B) To serve as a positive control by demonstrating that a protein in the extract is the transforming factor (C) To serve as a negative control by demonstrating that transformation does not occur without DNA (D) To destroy enzymes in the extract (E) To destroy any capsules that might be in the extract

Questions 14-16 are based on the following. In the 1940's, Avery, Macleod, and McCarty transformed nonencapsulated bacteria into encapsulated. forms by growing the nonencapsulated cells in a culture containing an extract made from dead encapsulated cells. The transformed cells produced colonies of encapsulated bacteria. Three different procedures and their results are outlined below. Procedure I: Extract made from dead encapsulated cells added to culture medium. Nonencapsulated bacteria added to culture medium. Results: Both nonencapsulated and encapsulated bacteria grow. Procedure II: Extract made from dead encapsulated cells treated with protein-degrading enzymes before adding extract to culture medium. Nonencapsulated bacteria added to culture medium. Results: Both nonencapsulated and encapsulated bacteria grow. Procedure III: Extract made from dead encapsulated cells treated with DNAse (an enzyme that selectively destroys DNA) before adding extract to culture medium. Nonencapsulated bacteria added to culture medium. Results: Only nonencapsulated bacteria grow. 14. A reasonable conclusion to draw from the results of the experiment is that (A) DNA is the genetic material (B) DNA replication is semiconservative (C) DNA is a double helix (D) DNA is translated into protein (E) mutation is a change in the genetic material 15. What was the purpose of treating the extract with protein-degrading enzymes in Procedure II? (A) To demonstrate that the transforming factor is an enzyme (B) To demonstrate that the transforming factor is not a protein (C) To destroy nucleic acids in the extract (D) To destroy any capsules in the extract (E) To prevent the extract from being contaminated by nonencapsulated bacteria 16. What was the purpose of treating the extract with DNAse in Procedure III? (A) To remove the encapsulated bacteria from the extract (B) To serve as a positive control by demonstrating that a protein in the extract is the transforming factor (C) To serve as a negative control by demonstrating that transformation does not occur without DNA (D) To destroy enzymes in the extract (E) To destroy any capsules that might be in the extract

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter12: Gram-positive Cocci

Section: Chapter Questions

Problem 4TBP

See similar textbooks