What is the best descriptive match for the following culture media? A. Specific biological characteristics allow this media to select specific groups of bacteria. B. All the components are well known. C. Will contain components that can inhibit certain groups of bacteria. D. Can be used to grow difficult growing or nutritional complex bacteria. E. This media contains amino acids for growing bacteria. select 1. Synthetic media select - 2. Selective media select - 3. Enriched media select 4. Differential media select - 5. Complex media

What is the best descriptive match for the following culture media? A. Specific biological characteristics allow this media to select specific groups of bacteria. B. All the components are well known. C. Will contain components that can inhibit certain groups of bacteria. D. Can be used to grow difficult growing or nutritional complex bacteria. E. This media contains amino acids for growing bacteria. select 1. Synthetic media select - 2. Selective media select - 3. Enriched media select 4. Differential media select - 5. Complex media

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 2CR

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1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?
THIS IS A 2 PART Question a. Is this a good streak? If so, explain why. If not, explain what you would change. b. Is this streak of a pure culture, a mixed culture, or a contaminated culture? Explain your response.
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.
After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.
You have been directed to take a sample from a growth-free portionof the zone of inhibition in the Kirby-Bauer test and inoculate itonto a plate of nonselective medium.a. What does it mean if growth occurs on the new plate?b. What if there is no growth?
A microbiology student was given a mixed culture of two different gram positive bacteria species to grow into a culture medium using correct aseptic techniques. After two days,one gram positive bacterial species grew on the media and the growth appeared red on the surface of the medium. Tthe second gram positive bacterial species grew and the growth appeared yellow on the surface of the medium. What is the possible explanation for the differences in the color of the bacterial growth? A. the culture was contaminated B. the microbiologist put too much inoculum on the culture medium C. the medium was a selective medium D. the medium was differential
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation
You pipette 1mL from a 30mL starting culture into a 99mL blank labeled A. You plate 1mL and 0.1mL of blank A onto plates B and C, respectively. Plate B has 310 colonies and Plate C has 31 colonies. What is the total cfu of the original culture? Select one: a. 9.3e5 b. 3.1e3 c. 3.1e4 d. 9.3e4 e. 3.1e5
What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contamination
Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
The isolation of Salmonella Typhimurium and S.Typhi require different protocols: A) In what aspects do these protocols differ? B) why do these protocols differ? C) How will you be able to distinguish between these organisms on XLD and Hektoen Agar?