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. Consider the chromatogram shown below (used with permission from the Honors Thesis of Mark Bickley, TU Chemistry alumnus). The chromatography column used to separate the three analytes (A, B, and C) is 17.0 cm in length. The elution time for an unretained solute is 2.05 min. B. Time (min) a) Calculate the adjusted retention time for compound B. b) Calculate the retention factor for compound B.

. Consider the chromatogram shown below (used with permission from the Honors Thesis of Mark Bickley, TU Chemistry alumnus). The chromatography column used to separate the three analytes (A, B, and C) is 17.0 cm in length. The elution time for an unretained solute is 2.05 min. B. Time (min) a) Calculate the adjusted retention time for compound B. b) Calculate the retention factor for compound B.

Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter31: Introduction To Analytical Separations
Section: Chapter Questions
Problem 31.32QAP
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9. Consider the chromatogram shown below (used with permission from the Honors Thesis of Mark Bickley, TU Chemistry alumnus). The chromatography column used to separate the three analytes (A, B, and C) is 17.0 cm in length. The elution time for an unretained solute is 2.05 min. 25 T0 7.5 100 Time (min) a) Calculate the adjusted retention time for compound B. b) Calculate the retention factor for compound B. c) What is the width (in minutes) at half-height of the peak associated with compound B? d) Based on your results from part c, how many plates are experienced by compound B? e) Based on your results from part d, what is the plate height (in um)? f) Calculate the unadjusted relative retention associated with compounds B and C. g) Calculate the relative retention associated with compounds B and C. h) Calculate the resolution associated with compounds B and C.
Transcribed Image Text:9. Consider the chromatogram shown below (used with permission from the Honors Thesis of Mark Bickley, TU Chemistry alumnus). The chromatography column used to separate the three analytes (A, B, and C) is 17.0 cm in length. The elution time for an unretained solute is 2.05 min. 25 T0 7.5 100 Time (min) a) Calculate the adjusted retention time for compound B. b) Calculate the retention factor for compound B. c) What is the width (in minutes) at half-height of the peak associated with compound B? d) Based on your results from part c, how many plates are experienced by compound B? e) Based on your results from part d, what is the plate height (in um)? f) Calculate the unadjusted relative retention associated with compounds B and C. g) Calculate the relative retention associated with compounds B and C. h) Calculate the resolution associated with compounds B and C.
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