. In an interrupted-conjugation experiment in E. coli, thepro gene enters after the thi gene. A pro+ thi+ Hfr iscrossed with a pro- thi- F- strain, and exconjugants areplated on medium containing thiamine but no proline. Atotal of 360 colonies are observed, and they are isolatedand cultured on fully supplemented medium. These cultures are then tested for their ability to grow on mediumcontaining no proline or thiamine (minimal medium),and 320 of the cultures are found to be able to grow butthe remainder cannot.a. Deduce the genotypes of the two types of cultures.b. Draw the crossover events required to produce thesegenotypes.c. Calculate the distance between the pro and thi genesin recombination units.
Bacterial Genomics
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Transformation Experiment in Bacteria
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Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
. In an interrupted-conjugation experiment in E. coli, the
pro gene enters after the thi gene. A pro+ thi+ Hfr is
crossed with a pro
- thi
- F
- strain, and exconjugants are
plated on medium containing thiamine but no proline. A
total of 360 colonies are observed, and they are isolated
and cultured on fully supplemented medium. These cultures are then tested for their ability to grow on medium
containing no proline or thiamine (minimal medium),
and 320 of the cultures are found to be able to grow but
the remainder cannot.
a. Deduce the genotypes of the two types of cultures.
b. Draw the crossover events required to produce these
genotypes.
c. Calculate the distance between the pro and thi genes
in recombination units.
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