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- How does cloning be helpful? Cite specific field or areas where cloning is beneficial. Explain in 10 sentences, please.Can I get a paragraph about the Repo protein that is used in Drosophila melangaster?Prior to the work described here, what was the original purpose ofMorgan’s experiments with Drosophila?
- Suppose you had funding to do a genome-wide gene expression experiment (20,000 genes). You can use a total of 20 RNA samples. Briefly describe the experiment you would run. In the context of your experiment, what is a false positive? In the context of your experiment, what is a false negativeYou find a new gene and want to know if it is expressed in the nervous system of Drosophila.What do you think is the quickest method to test this?Please answer all questions if possible. What do loss of function alleles tell us about normal gene function? Why would a researcher be interested in over- or mis-expression phenotypes? Why are genetic screens useful? Why do biologists do screens? What are we trying to do? What is the Big Picture purpose?
- The first attempts at gene therapy began in 1990 with the treatment of a young girl with a genetic disorder abbreviated SCID. What does SCID stand for? In the context of SCID, what does ADA stand for?When doing a lab that involves Extraction of Genomic DNA from adult Drosophila melanogaster. - What are the controls you should use and why use these? What is special about the genomic DNA? What do you expect to find (using these genomic DNA samples)? please help!Explain how you would compare the expression of a gene in a cancer cell and a normal cell using qPCR, a DNA chip, and RNA-seq analysis.