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- An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding region of his DNA reads ATGGGGATATGGCAT. If the protein-coding region of a healthy adult reads ATGGGGATATGAGCAT, identify the site and type of mutation.Which subunit of the E. coli polymerase confers specificity to transcription? a. b. c. d.1. a)What would happen if the aminoacyl tRNA synthetase responsible for charging alanine tRNAs also charged methionine tRNAs with alanine? b)What would happen if an individual was homozygous for mutant alleles of the gene encoding the aminoacyl tRNA synthetase responsible for charging leucine tRNAs?
- 1. Explain why do eukaryotic mRNAs have to be “processed” whereas most prokaryotic RNAs do not?1. What is Transcriptome? What is Transcriptome analysis? How does the method yield information able to elucidate the structural apexes of the molecules under examination? What are the steps and primary methods used in Transcriptome analysis? What are some important applications of Transcriptome analysis?Please be as detailed as you could be in your explanation.1. Why aren't ssb proteins necessary in transcription?
- 1. Germline genetic editing is treated with great caution by scientists, what are the potential risks of germline genetic editing and if one day, germline gene editing were to become legal, what criteria do you think will have to be met first?32. All of the following are functions of introns EXCEPT A. Provide buffering capacity against mutations B. Allow shuffling of exons to make new genes C.Allow for alternative splicing, which allows a single gene to encode multiple proteins D. Allow for alternative splicing, which allows multiple genes to encode a single protein1. Classify the type of mutation that have taken place: silent, missense and nonsense as a result of a single base substitution from UCG codon which codes for cysteine:a) AGC (ser): ________b) UGU (cys): ________c) GGC (gly): _______d) UGA (stop): _______e) UUC (phen): _______2. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacterial virus T4 caused a change in the protein fromits wil-type composition….lys-ser-pro-ser-leu-asn-ala-ala-lys…..to the mutant form lys-val-his-his-leu-met-ala-alalys.a. Decipher the segment of mRNA for both the original protein and the double mutant.b. Which base was added? Which was deleted?3. Given is the 30 nucleotides in the human gene for hemoglobin (the oxygen-carrying protein in the red blood cells): 5’ TAC-CAC-GTG-GAC-TGA-GGA-CTC-CTC-TTC-AGA 3’a. What is the complementary strand?b. Deduce the mRNA in this coding region.c. What is the amino acid sequence based on this…