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- Assume that the molar percentage of thymine in a double stranded DNA is 20. What are the percentages of the four bases (G, C, T, A)? b. The base content of a sample of DNA is as follows: A=31% G=31% T=19% C=19%. What conclusion can be drawn from this information?The amount of G plus C in an organism’s DNA is 64% of the total base content of that DNA. What are the percentages of A, T, G, and C?You add 1 ul of EcoRI into 50 ul of your reaction of which contains 10 ug of DNA. How long would it take to completely digest the DNA?
- You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionThe Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your answers, show how you came up to each result?(a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in micrometer?If you have G-C rich DNA versus A-T rich DNA. (a)Will the melting temperature be the same? Why? (b) Will the annealing temperature be the same? (c) Which of the categories in (a) above is more prone to errors?
- If you have G-C rich DNA versus A-T rich DNA. Will the melting temperature be the same? Why? Will the annealing temperature be the same? Which of the categories in (a) above is more prone to errors?"Chargaff's rules" about the composition of bases in DNA dictates that A. the sum of purine residues must equal the sum of pyrimidine residues. B. the sum of A-T base pairs must equal the sum of G-C base pairs. C. the base composition of DNA is the same in all species. D. DNA specimens isolated from different tissues of the same species vary in base composition.You perform an experiment to determine the complete melting profile of a relatively long DNA duplex with 80% G:C base pairs. Sketch a graph of Absorbance at 260 nm (Y-axis) vs. Temperature (X-axis). Label the location of the Tm and describe what is happening to the DNA structure in each segment of the graph (lower Temps, highest Temps, at Tm).
- In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.Which of the following is NOT true of deoxyribonucleic acid (DNA)? A. None of the other four answers (all are true of DNA) B. Each DNA nucleotide contains the sugar ribose, phosphate, and one of the following nitrogenous bases: adenine, uracil, guanine, or cytosine C. Stores genetic information that can be expressed within a bacterial cell, transferred “vertically” to offspring cells, or “horizontally” to other recipient cells D. Found as a circular molecule in bacteria, either as chromosomes or plasmids E. Serves as a template for the transcription of mRNAIn a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the DNA structure. What is the fundamental physical basis for the absorbance increase? a. the effective concentration of DNA changes b. the absorbance of DNA is highly sensitive to temperature c. native DNA absorbs light more strongly than denatured DNA because the bases are stacked d. denatured DNA absorbs light more strongly than native DNA because the bases have become unstacked