Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.8: Fecal Occult Blood Test
Problem 1CT
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Transcribed Image Text:Collect evidence from
6)
the crime scene
Probe the membrane
with DNA fragments
that complement
the DNA sequence
of the fragments of
interest.
Compare the fragment profile of
the evidence DNA with those of the
suspects, detective and victim to
see if they match.
2
Isolate DNA from an
evidence sample
3
Cut the DNA into fragments using
specialized protein "scissors" called
restriction enzymes. For every person,
the sizes of the cut fragments are
unique - except for identical twins.
4
Separate the negatively
charged DNA frag-
ments in a gel
by passing an
electric current
8
Re-probe the membrane up to
10 more times to identify different
fragments.
GEL
through it.
If the profiles from the evidence
DNA and a suspect match multiple
times,then it is very likely that the
Transfer the DNA
fragments from
the gel to a sheet
evidence DNA came from the
suspect.
of membrane
1.
Describe 3 things that you notice about the
process of Gel electrophoresis in the diagram
above.
1.
2.
3.
2. How are DNA profiles compared to detect
possible contamination of samples?
Evidence
DNA
Suspect #1
Suspect #2
| Victim
Officer
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