1- What are the main two types of columns in the HPLC? 2-What are the most disadvantages of mass-spectrmeter as a detector for HPLC? 3-What Are The Commonly Used Carrier Gases In GC Analysis ? 4-Name four of the mechanisms of the separation in chromatography? 5-Which Is The Most Commonly Used Detector In High Performance Liquid Chromatography And Why?

Q: What are the similarities and differences between a chromatogram from an HPLC with a diode array…

A: A chromatogram formed after chromatography technique.  A chromatography is a technique to separate…

Q: If you perform a column chromatography based on the present TLC analysis, which compound is eluted…

A:

Q: The identity and ratio of solvents are necessary in chromatography for achieving a good separation.…

A: if your solvent had an overly polar TLC developer then all the components of the mixture will move…

Q: How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your…

A: A 2D - TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid…

Q: Explain shortly how the chromatography will be influenced in a C18 reverse phase liquid…

A: “Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…

Q: Which statements are true for molecular exclusion chromatography?I            Molecular exclusion…

A: ✓As the name ,Size-exclusion chromatography, also known as molecular sieve chromatography, is a…

Q: Which statements are true for molecular exclusion chromatography? I            Molecular exclusion…

A: Molecular sieve chromatography, is a chromatographic process that separates molecules in solution…

Q: ultra-high-pressure

A: Ultra–high–pressure liquid chromatography – Ultra high-pressure liquid chromatography is the same as…

Q: n lab two different mobile phase compositions (acetone and hexanes) were used for column…

A: The solution of the question is given below:

Q: b. Describe the THREE major classification of Chromatography b. Two compounds in gas chromatography…

A: Chromatography is a useful technique to precisely separate, analyse, and purify a wide range of…

Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…

A:

Q: What are the principles involved in the separation of substances in Thin Layer Chromatography? 2.…

A: The question is based on the concept of organic reactions. We have to explain the principle of thin…

Q: Choose the correct option. Which one of following techniques is used as fractionation as well as…

A: Molecular weight is the mass of one mole of substance. Units of molecular weight is grams per mole.…

Q: What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for Analysis of…

A: a). HILIC. Because there will be occuring hydrophilic intraction chromatography also it can partly…

Q: 2. In HPLC analysis, why Would peak area is preferred to peak height in evaluating the caffeine…

A: Since you have asked multiple questions, we will answer the first question for you. To get the…

Q: Which of the following is TRUE for a reversed phase chromatography experiment? i. Less polar…

A:

Q: Suggest a reason why the optimum linear flow rate is much higher in gas chromatography than in…

A: Given:-             The optimum linear flow rate is much higher in gas chromatography than in liquid…

Q: Classify the stationary phases based on whether they are commonly used in reversed-phase liquid…

A:

Q: A student places an unknown sample on a thin-layer chromatography plate and develops it in…

A: The important points in the question are: An unknown sample was placed on a thin-layer…

Q: 2. Do compounds always elute in the same order on a TLC plate and on chromatography column? Why or…

A: TLC stands for thin layer chromatography

Q: Describe how a TLC study could be used to determine an appropriate solvent system for a column…

A: TLC is thin-layer chromatography and its a technique to separate non-volatile mixtures and identify…

Q: What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for Analysis of…

A: a). HILIC. Because there will be occurring  hydrophilic interaction chromatography also it can…

Q: What is the principle behind reverse-phase ion-pairing chromatography?

A: Reverse Phase ion chromatography use the principle of  Solute ions instead of molecules .

Q: What is the objective of the Separation Technique: Paper Chromatography ?

A: A separation process is a method that converts a mixture or solution of chemical substances into two…

Q: Which of the following is TRUE for a reversed phase chromatography experiment?    i. Less polar…

A:

Q: Explain the difference between wall-coated, support-coated, and porous-layer open tubular columns…

A: In wall coated columns the stationary liquid phase rests on capillary column which in turn consists…

Q: Indicate 3 uses of Thin Layer Chromatography  What are the stationary and mobile phases of this…

A: Introduction :  Thin layer chromatography is a technique which is used for the separation of…

Q: Define each of the following terms: a. Thermal gradient, b. isocratic elution Describe some ways to…

A: “Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: Rank the following compounds in terms of decreasing theoretical retention factors if a reverse-phase…

A: Reverse-phase chromatography is a chromatographic technic in which the stationary phase is…

Q: _is a chromatography technique in which the stationary phase 14. is always lon exchange resin."…

A: Chromatography is a technique in which we separate a single chemical compound from a mixture. If the…

Q: 04. Describe in detail the five basic steps in the thin layer chromatographic (TLC) separation.

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: What are the quantification methods for supercritical fluid chromatography?

A: Interpretation- To give all the quantification methods for supercritical fluid chromatography -…

Q: For thin layer chromatography, why should a piece of filter paper that is moistened with the…

A: Purpose of the Filter Paper in the Thin-Layer Chromatography :- Filter paper is placed in the…

Q: 1) The efficiency of a GC separation of 2-butanone on a dinonyl phthalate packed column was studied.…

A:

Q: planar chromatography and what type of different planar cgromatographic methods are available

A: The planar chromatography is a technique used to separates mixture of the compounds. The form of…

Q: What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography…

A: There was so many disadvantages of uses of  thick layer stationary phase in thin layer…

Q: Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result…

A: Thin-layer chromatography has two phases a stationary phase and a mobile phase. The silica gel…

Q: what is the advantage of TLC as compared to the column chromatography ?

A: Welcome to bartleby !

Q: Explain in details the basis and essence of High Performance Liquid Chromatography (HPLC) method of…

A: HPLC (High-performance liquid chromatography) HPLC Chromatography: Chromatography is a technique it…

Q: How can you ensure “good quality” peak separation in high performance liquid chromatography (HPLC)?

A: In HPLC , different kind of columns are used to separate to isomers. So, here by using trial method…

Q: is hexane or 70:30 hexane:  ethyl acetate a better eluent based on their TLC plates and rf value in…

A: Misciblity is main factor which determine that which solvent are best eluent  based on their TLC…

Q: What is the purpose of solid-phase extraction? Why is it advantageous to use large particles (50 mm)…

A: An extraction method where compounds are suspended (or) dissolved in mixture of liquid are isolated…

Q: Ion exchange chromatography can be used to separate a mixture of cations and anions, true or false?

A: Chromatography is the method used in laboratories for the separation of elements or molecules.

Q: enumerate the advantage of TLC methods of chromatography over column and paper chromatography.

A: Chromatography is a useful technique to precisely separate, analyse, and purify a wide range of…

Q: With the aid of a well labelled block diagram, briefly describe the functions of the major…

A: When two or more compounds are mixed together in a specific ratio/random way, it results in the…

Q: . What factors can affect a solute's Rf value in thin-layer chromatography? 2. Why is it necessary…

A: Answer - According to the question - Rf values and reproducibility can be affected by a number of…

Q: thin layer chromatography: What would happen to the Rf value of a compound if you increased the…

A: One of the methods of identification of the individual compounds from a mixture of compounds is TLC.…

Q: Do compounds always elute in the same order on a TLC plate and on a chromatography column? Why or…

A: Once in a while, rather than letting the eluent go through the segment by means of gravity, the…