Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed:Rf Values: (A: 0.6, B: 0.9, C: 0.5, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method?2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the ?meaning of RT
Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed:Rf Values: (A: 0.6, B: 0.9, C: 0.5, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method?2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the ?meaning of RT
Chapter88: Column Chromatography
Section: Chapter Questions
Problem 4P
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Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed:Rf Values: (A: 0.6, B: 0.9, C: 0.5, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method?2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the ?meaning of RT
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