1-What can occur if you quickly drop the coverslip onto your wet mount? 2-How can one add stain to the wet mount that has already been prepared? 3-Some live microorganisms can move very quickly. How do we slow these down to view in the microscope?
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- 1. You're using a microscope with 10x magnification ocular lenses and a 25x magnification objective lens. What is the microscope's overall magnification at this setting? 2. Is it correct that immersion oil improves image magnification and resolution?The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?If you gram stain an acid fast organism, what would you most likely see when you examine the stain under oil immersion? You are in a hurry to test a bacterial culture for spore production. You grow the culture or 12 hours and then stain it. Your results are negative. Should you trust your results? Why or why not?
- 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?A new lab recruit performs their first Gram stain on a slide containing both G(+) and G(-) organisms. After finishing the staining, they realize that they misread the labels on the crystal violet and Gram’s iodine vials. They accidentally used Gram’s iodine in place of crystal violet, and vice versa. Explain what you expect the recruit to see under the microscope, and explain why they see it.1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?
- 1. Is a microscope an absolute necessity for studying microorganisms? Why or why not? 2. What do you think are the limitations of a brightfield microscope? Why is a brightfield microscope the most common type of microscope in routine clinical laboratories? 3. A slide in which a stained specimen is placed on the microscope stage. After careful and correct manipulation of the adjustment knobs, the specimen is still not in focus. What do you think is the reason? What corrective actions can be done? (provide computation) 1. What is the formula to determine the actual size of an organism? 2. The actual size of an Escherichia coli is 1.5um. What would be its image size if the magnification used is 1000x? 3. What magnification is used if an Bacillus sp. have a microscopic size of 3.5um and an image size of 15mm? NOTE: please try to answer all of the question, i promised to give you a good ratingg1. What does moving the coarse focus and fine focus do? Why is it important to remember the difference between coarse and fine focus? 2. What is the resolution of a microscope? How is resolution affected byadjusting the light, coarse and fine focus? please answer on ur own words, do not copy from google or i will downvoteThe image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification used on the plant and animal photos. Why are the bacterial cells so much harder to see in this microscope image?
- 1. You accidentally used safranin as the primary stain and malachite green as the counter stain during a spore staining procedure. Explain how your microscopic observation would differ from those observed when slides of spore-containing bacteria were prepared correctly. 2.One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found? What errors could have contributed to the variable result?Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorization1. Name the part of the microscope that you use to control the cone of light coming through the condenser. 2. What is the total magnification of your specimen you are viewing using the 40X lens?