Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A:
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: Which of the following are true about a differential stain. There may be more than one answer!…
A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…
Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Match the organism with the method of staining best used to identify it. Salmonella Typhi…
A: Stain It is a substance that adheres to a cell, giving the cell color. The presence of color gives…
Q: Why is it necessary to use a 24-hrold culture in gram staining?
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…
A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…
Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…
A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…
A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…
Q: b. Successfully completed most steps of the Gram stain but failed to add the iodine (the mordant):…
A: Gram staining is a differential staining procedure used to classify two types of bacteria on the…
Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…
A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
A: Spores are cellular structures produced by plants, bacteria, and fungi. Spores can be produced for a…
Q: Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary…
A: Differential staining uses more than one type of stains for staining the microorganisms and it is…
Q: With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What…
A: Gram stain Gram staining is a method of staining which is used to classify the bacterial species…
Q: list 4 advantages and 4 limitations of the Gram staining process
A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…
Q: Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please…
A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…
Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…
A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…
Q: The decolorizer used in the Gram Stain, and the decolorizer used in the Acid Fast Stain, are the…
A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…
Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…
A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a…
A: NOTE: AS PER OUR GUIDELINES WE ARE ONLY ALLOWED TO ANSWER FIRST QUESTION. PLEASE RESUBMIT THE OTHER…
Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…
A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…
A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…
Q: 1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: What is Gram staining?Describe step by step the procedure.
A: GRAM STAINING METHOD:- This is a differential staining that was developed by Dr. Hans Christian Gram…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…
A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…
A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…
Q: Out of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast…
A: The unknown bacteria can be identified by morphological and biochemical methods. The method of…
Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…
A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…
Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…
A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…
Q: ) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step…
A: On the basis of lipid content and peptidoglycan layer in the cell wall, bacterias are classified…
1.)What is the purpose of a counterstain?
2. What does a mordant do in the
3. True or False? The oil objective should make contact with the oil on the slide.
4. Why is it necessary to let bacterial smears completely air dry before heat fixing?
5.Why should controls be included wherever possible for any staining technique?
6. Why is it necessary to heat the slides while staining for endospores?
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few seconds
- Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 XWhat is the purpose of each reagent in gram-staining? Primary stain Mordant Decolorizing agent Counterstain1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed to make a simple stain of coli with crystal violet, but got mixed up and used safranin instead. How would their observations be different? Would the information obtained be any different? 3-How is the procedure different when taking cells from a solid medium compared to taking cells from a liquid medium? Why is it important that your smear be thin?
- You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect?What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other choices To rinse off excess reagent To remove unstabilized crystal violet To act as a mordant
- Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please explain, thank you.Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Testwhy are the steps in gram staining are so carefully standardized?