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- 4c. Calculate the values of Mn, Mw, Mz and polydispersity index for a polymer mixture having equivalent moles of two polymers with exact molecular weight being 2,000 Dalton and 10,000 Dalton.Outline methods for polymer molecular weight determination by dilute solution viscosity measurement.Introduction for polymer molecular weight determination by dilute solution viscosity measurement.
- The absorbance of a 0.5 mM aqueous salicylic acid solution was 0.925. This solution was extracted with 10% ethyl acetate/hexane solution. The absorbance of the extracted aqueous salicylic acid solution at equilibrium was 0.670. Find the equilibrium concentrations of SA in the aqueous and the non-aqueous phases. Calculate the partition coefficient (K) for the salicylic acid.Explain the difference in nephelauxetic effect of the Racah parameter (B) for [Co(CN)6]3- = 460 cm-1 and [Co(NH3)6]3+= 615 cm-1.what is Optical rotatory dispersion and circulatory dichroism method for protein binding?
- The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards on an SDS– polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass of a protein having a mobility of 0.62 on this gel?The volume of gas at 20 °C and 1.00 bar adsorbed on the surface of 1.50 g of a sample of silica at 0 °C was 1.42 cm3 at 56.4 kPa and 2.77 cm3 at 108 kPa. Assume that the Langmuir isotherm applies and estimate the value of V∞.In the food dye experiment, the molar absorptivity of Allura Red is 2.59 x 104 M-1cm-1, account for factors that may affect the accuracy of the modified spectrophotometric set up?
- Discuss the dependence of Mn and Mw on the presence of high and low molecular weight material.2. The onset of the glass transition temperature Tg of the following polymers was measured: • poly(n-butyl methacrylate) (CAS# 9003-63-8) • poly(methacrylic acid) (CAS# 25087-26-7) • poly(n-hexyl methacrylate) (CAS# 25087-17-6) • poly(methyl methacrylate) (CAS# 9011-14-7) • poly(t-butyl methacrylate) (CAS# 25189-00-8) The measured values, in decreasing order, were: 228oC, 115oC, 110oC, 20oC, and -5oC. Assign the correct Tg to each polymer. Draw the structure for each polymer to help explain your answer. Support and explain your answer in your own wordsGlycolic acid is used in the textile industry as a dyeing and tanning agent. Given that its acid dissociation constant (Ka) is 1.47 × 10-4 and its partition coefficient (KD) between water and benzene is 3.22, calculate the extraction efficiency of a single extraction when 50.00 mL of a 0.025 M aqueous solution of glycolic acid buffered to a pH of 3.00, is extracted once with 50.00 mL of benzene.