Answered: In the food dye experiment, the molar…

In the food dye experiment, the molar absorptivity of Allura Red is 2.59 x 104 M-1cm-1, account for factors that may affect the accuracy of the modified spectrophotometric set up?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.3QAP

See similar textbooks

Related questions

Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…

Q: Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are…

A: Hey, since there are multiple subparts posted, we will answer first three subparts. If you want any…

Q: In TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so…

A: Thin-layer chromatography (TLC)-It is a technique used for the separation of a mixture of volatile…

Q: What is the importance of injecting analytes separately in order to ascertain the elution order in…

A: Gas chromatography is a common type of separation method and analysis of chemical compounds that can…

Q: Micellar electrokinetic capillary chromatography was conducted at pH 10 with anionic micelles and…

A: (i) In micellar electrokinetic capillary chromatography, surfactants are added to the buffer…

Q: An analyte is found to elute from a reversed-phase column with a retention factor of 12.5 when a 20%…

A: Given data :- At 20% ethanol and 80% water concentration retention factor = 12.5

Q: What effect will the following factors have on a chromatographic separation? (a) Too strong an…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: State the functions for each of these components used in the gel filtration chromatography using a…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: Is it possible to detect metal complexes of organic acids with thin layer chromatography?

A: Thin layer chromatography, abbreviated as TLC, is used to identify the element or compound present…

Q: Rank the following compounds in terms of decreasing theoretical retention factors if a reverse-phase…

A: Retention factor is the ratio of distance travelled by the sample to that of distance travelled by…

Q: (3) A 0.35 mm diameter open tubular gas chromatography column is coated with stationary phase that…

A: Golay's equation Golay's equation is used to find the separation efficiency of open tubular column.…

Q: Species A and B were separated using column chromatography. The elution time for species A (1) was…

A: The theoretical plate height H = L/N where L is the length of the column and N = (tr)2/(w/4)2 where…

Q: A) What pH should the solvent system be to perform TLC on a sample suspected of containing…

A: A) Because the putative molecule is slightly acidic, we must utilize a solvent solution that is very…

Q: 1. The following data are for a liquid chromatographic column: length of packing: 24.7 cm VM: 1.37…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385 s,…

A: The solution of the question is given below:

Q: What could be the factors that contribute to the inaccuracy or the modified spectrophotometric…

Q: wo components in an HPLC separation have retention times that differ by 22 s. The first peak elutes…

Q: density

A: According to the question, the readings are: 0.24, 0.35, 0.67, 0.89. We need to determine the…

Q: PART A: Substance A was found to have a retention times of 3.15 minutes on a 70.00-cm column. An…

A: Hello. Since the question contains multiple subparts, the first three parts are solved. In case the…

Q: 2. In HPLC analysis, why Would peak area is preferred to peak height in evaluating the caffeine…

A: Since you have asked multiple questions, we will answer the first question for you. To get the…

Q: Suggest a reason why the optimum linear flow rate is much higher in gas chromatography than in…

A: Given:- The optimum linear flow rate is much higher in gas chromatography than in liquid…

Q: ii) What are the adjusted retention times for components A and B? iv) What is the relative retention…

A: 3 . For component A = 8-1.2=6.8 For component B = 10-1.2 = 8.8 4. Separation factor = 1.29

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 24.8 cm. The…

A: Given : Separation resolution between A and B can be calculated using formula: Rs = 2RtB-RtAw1 +…

Q: Explain what will happen when you use 100 microgram of an analyte in a chromatography column which…

Q: (a) Use the information given below to sketch and label a molecular exclusion calibration curve. Use…

Q: What is the HETP of a column that is 200mm with a retention time of 5mins. The (effective)…

A: Separation factor is calculated as: a=tr-tmtm where tr is the retention time and tm is the dead…

Q: The following data were obtained for four compounds separated on a 20-m capillary column. t, (min) w…

A: Number of theoretical plates indicates the column efficiency meaning that higher plate numbers…

Q: Choose the best answer. What are the factors that need to be considered in HPLC to ensure the two…

A: Introduction : HPLC means High pressure liquid chromatography it is used to find out the drug…

Q: The separation of APAP-L,(12 = 0.28 min) from APAP-D (W12 = 0.35 min) on a chiral stationary phase…

A: APAP-L W12 = 0.28 min has a retention time of 4.78 min and APAP-D W12 = 0.35 min has aretention…

Q: The following data were obtained by gas-liquid chromatography on a 40-cm packed column: Compound tr,…

A: To solve this problem, we will need the following relations regarding gas-liquid chromatography : 1.…

Q: For a chromatographic peak, what is the value of retention time of analyte tR if retention time of…

A: Correct Option : A. 75.15 s

Q: Distance travelled by solvent: 5.50cm TCL pure caffeine Distance travelled by the component: 2.20cm…

A: The retention factor or the Retardation factor or in other words delay factor is a ratio of the…

Q: What information is displayed in a reconstructed total ion chromatogram, selected ion monitoring,…

Q: Which mechanisms of band broadening that operate in chromatography are absent in capillary…

A: The process of band broadening is basically explained by the Van Deemter equation in chromatography…

Q: Define each of the following terms: a. Thermal gradient, b. isocratic elution Describe some ways to…

A: “Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: Water and methanol are separated using a 1.0-m packed gas chromatography column. An unretained…

A: The linear velocity of the mobile phase is calculated with the help of equation (1) where L is the…

Q: The following data were obtained for four compounds separated Compound on a 20-m capillary column.…

A: Hello, For calculating selectivity factor, the value of tM that is the elution time of an…

Q: Briefly explain how analytes are separated using the following modes of chromatography.…

A: Chromatography is a process in which a chemical mixture is carried by a liquid or gas as medium is…

Q: (3) A 0.35 mm diameter open tubular gas chromatography column is coated with stationary phase that…

A: Gas chromatography (GC) is a technique that is used for the quantitative analysis of organic…

Q: Calculate the separation factor (or selectivity factor), between two compounds, 1 and 2, whose…

A: The selectivity factor (α) of a column for two analytes A and B where A eluting before B is given…

Q: You are trying to separate a sample with 2 components: X and Y with GC. X has a lower boiling point…

A: Chromatography techniques are used to separate two r more compounds. The carrier gas is the mobile…

Q: Explain what the following factors consist of and their influence on the chromatographic…

A: Chromatography is a technique of separation and isolation of sample compounds. It works on the…

Q: The following data are for a GLC column: L= 35.5 cm, flow rate 0.303 mL/min A chromatogram of a…

A: Hello. Since the question contains multiple subparts, the first three parts are solved. In case the…

Q: enumerate the advantage of TLC methods of chromatography over column and paper chromatography.

A: Chromatography is a useful technique to precisely separate, analyse, and purify a wide range of…

Q: With the aid of a well labelled block diagram, briefly describe the functions of the major…

A: When two or more compounds are mixed together in a specific ratio/random way, it results in the…

Q: culate the selectivity factor (a) for the peak pairs of 1,2 and 3,4 and 5,6 in the chromatogram…

Q: True of false? 1. Raman Spectroscopy can be used in the airport to detect the presence of Alcohol in…

A: To find : True or false. Solution : We will look all the statement one by one.

Q: Which of these is NOT an electrochemical measurement technique? O chronopotentiometry O column…

Q: Identify the mobile and stationary phases in thin layer chromatography (TLC) and explain how the…

A: Chromatography: Chromatography is a kind of laboratory process that is used in the separation of…

Question

In the food dye experiment, the molar absorptivity of Allura Red is 2.59 x 104 M-1cm-1, account for factors that may affect the accuracy of the modified spectrophotometric set up?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

The molar absorptivity of Allura Red is 2.59 x 104 M-1 cm-1, account for the accuracy of the modified set-up. What could be the factors that contribute to the inaccuracy or the modified spectrophotometric set-up?
The predominate form of caffeine at pH 9.4 is neutrally charged. If nitromethane, a compound often used as neutral marker, was added to the sample and standard CE vials, predict the impact on the electropherogram, the peak areas, and the determination of caffeine, aspartame and benzoic acid concentrations?
What could be the factors that contribute to the inaccuracy of the modified spectrophotometric set-up?
The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards on an SDS-polyacrylaide gel are 0.80 and 0.41. What is the apparent mass of a protein having a mobility of 0.62 on ths gel?
Micellar electrokinetic capillary chromatography was conducted at pH 10 with anionic micelles and the anode on the sample side. (i) Is aminobenzene (C6H5NH2) a cation, an anion, or a neutral molecule in this experiment? (ii) Explain how to decide which compound, aminobenzene or anthracene, is more soluble in the micelles.
In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?
Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16.They are separated by elution on silica gel with hexane as eluent. The ratio for the packingVS/VM =0.425a. Calculate the retention factor for each soluteb. Calculate the selectivity factorc. How many plates are needed to provide a resolution of 1.5?d. What column length should be used to provide a resolution of 1.5 if the plate height ofthe packing is 1.5 ×10-3cm?e. If the flow rate is 6.75 cm min-1, how long will it take to elute the two species? (the question wanted to be answered)
A) What pH should the solvent system be to perform TLC on a sample suspected of containing noscapine( pka= 6.7)The solvent system contains ethyl acetate and an aqueous portion.The TLC plate is bare silica.B) Suggest what specific reagent or reagents you would use to produce the above pH?
The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards on an SDS– polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass of a protein having a mobility of 0.62 on this gel?
Under what wavelength (in nm) should a spectrophotometer be set to be able to measure the optical density of microbial cultures? What could be viable reference blanks we can use to zero our spectrophotometer when measuring the optical density of microbial cultures?
an analytical separation technique based in the large differences in solubility between interference and analyte 1. Separation by extraction 2. Chromatographic separation 3. Separation of species by distillation 4. Separation by precipitation
Variation in volume of extraction solvent: compound X is dissolved in 20.0 mL of solvent B. Assuming a partition coefficient of PX,AB of 5.0, calculate the percentage of X that remains in solvent B if it is extracted the following volumes of solvent A: 1.0 mL 10.0 mL 20.0 mL 100.0 mL