1. at what wavelength was Pb determined? 2. What is the slope of the calibration curve? 3. What is the y-intercept of the calibration curve? 4. The researcher determined the Pb content of water sample from a river suspected to be contaminated with Pb from a nearby factory by complexing with cyanidine and the absorbance measured was 0.405. What is the lead content of the water sample

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1. at what wavelength was Pb determined? 2. What is the slope of the calibration curve? 3. What is the y-intercept of the calibration curve? 4. The researcher determined the Pb content of water sample from a river suspected to be contaminated with Pb from a nearby factory by complexing with cyanidine and the absorbance measured was 0.405. What is the lead content of the water sample?
Cyanidin is found in intensely colored flowers. A researcher complexed the flower extract to Lead (Pb)
and formed a deep purple color. Cyanidin is an eco-friendly ligand that can be used in UV-Vis analysis.
Instead of using toxic and expensive reagents, this was employed by the researcher. PbCl2 (278.1
g/mol) of a mass 0.090 g was used in the experiment. It was dissolved in 100.0 mL of distilled, de-
ionized water to prepare the stock solution. The X max was determined as follows:
A (Wavelength, nm)
400
420
440
460
480
500
520
540
A (Absorbance)
0.08
0.10
0.20
0.25
0.30
0.40
0.50
0.60
A (Wavelength, nm)
560
580
600
620
640
660
680
700
A (Absorbance)
0.50
0.40
0.30
0.25
0.20
0.10
0.05
0.03
The researcher made a standard calibration curve by diluting the 10.00 mL, 20.00mL, 30.00mL, 40.00
mL, and 50.00 mL solutions from the stock. Water was filled up to the 100-mL mark of the volumetric
flasks. The absorbance of the solutions were then obtained, and the data are shown below.
Concentration (ppm)
90.00
Absorbance
0.01
180.0
0.20
270.0
0.30
360.0
0.43
450.0
0.55
Transcribed Image Text:Cyanidin is found in intensely colored flowers. A researcher complexed the flower extract to Lead (Pb) and formed a deep purple color. Cyanidin is an eco-friendly ligand that can be used in UV-Vis analysis. Instead of using toxic and expensive reagents, this was employed by the researcher. PbCl2 (278.1 g/mol) of a mass 0.090 g was used in the experiment. It was dissolved in 100.0 mL of distilled, de- ionized water to prepare the stock solution. The X max was determined as follows: A (Wavelength, nm) 400 420 440 460 480 500 520 540 A (Absorbance) 0.08 0.10 0.20 0.25 0.30 0.40 0.50 0.60 A (Wavelength, nm) 560 580 600 620 640 660 680 700 A (Absorbance) 0.50 0.40 0.30 0.25 0.20 0.10 0.05 0.03 The researcher made a standard calibration curve by diluting the 10.00 mL, 20.00mL, 30.00mL, 40.00 mL, and 50.00 mL solutions from the stock. Water was filled up to the 100-mL mark of the volumetric flasks. The absorbance of the solutions were then obtained, and the data are shown below. Concentration (ppm) 90.00 Absorbance 0.01 180.0 0.20 270.0 0.30 360.0 0.43 450.0 0.55
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