1. Bacterial mutants that are unable to synthesize fatty acids will incorporate fatty acids into their membranes when fatty acids are supplied in their growth medium. Suppose that each of the two cultures contains a mixture of several types of straight-chain fatty acids, some saturated, and some unsaturated, ranging in chain length from 10-20 carbons. If one culture is maintained at 18°C and the other is maintained at 40° C over several generations, what differences in the composition of the cell membranes of the two cultures would you expect to observe? Explain your answer

1. Bacterial mutants that are unable to synthesize fatty acids will incorporate fatty acids into their membranes when fatty acids are supplied in their growth medium. Suppose that each of the two cultures contains a mixture of several types of straight-chain fatty acids, some saturated, and some unsaturated, ranging in chain length from 10-20 carbons. If one culture is maintained at 18°C and the other is maintained at 40° C over several generations, what differences in the composition of the cell membranes of the two cultures would you expect to observe? Explain your answer

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter6: Energy, Enzymes, And Biological Reactions

Section: Chapter Questions

Problem 8TYK: Which of the following statements about the allosteric site is true? a. The allosteric site is a...

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The bacteria that you are growing to express biochemisfunase are resistant to ampicillin, so you keep ampicillin in your media to prevent contamination from other bacterial strains. You find a tube of ampicillin in the lab freezer that says “2000x AMP” on the lid, which indicates that it is 2000 times more concentrated than what you need (i.e., a concentration of 1x ampicillin in your media). How much of the 2000x ampicillin stock would you add to your 1.0-L culture to get a final concentration of 1x ampicillin?
2 a. You are trying to purify protein C from a mixture of proteins noted in the above Table. If you had only one type of column to choose from, which one would allow you to purify protein C with the least number of contaminants? Size exclusion column Ion exchange column Affinity chromatography using glucose as the bait Affinity chromatography using NAD as the bait Please explain why you chose the column above based upon the properties of the column AND the proteins in the Table.
2. A mixture of the following amino acids (glu, leu, val, arg, ser, phe) was obtained upon complete hydrolysis of a hexapeptide. a. Edman’s reagent releases leucine b. Hexapeptide with carboxypeptidase releases serine. c. Hexapeptide with trypsin forms a tripeptide A with leucine at the N terminal and tripeptide B with valine at the N terminal. d. Tripeptide A with carboxypeptidase releases arginine and a dipeptide with glutamic acid at C terminal. e. Tripeptide B with chymotrypsin form releases serine and another dipeptide. Give: 1. the amino acid sequence of tripeptide A and B. 2. the amino acid sequence in the above hexapeptide.
I. How much of a 10 mg/mL lysozyme stock would you need to add to 4 mL of cells for a final concentration of 1 mg/mL? What is the lysozyme doing?
The answer given in problem 31. 17 is inadequate for me, and I simply don't understand the explanation for the answer... The problem is: Ribosoms were isolated from bacteria grown in a "heavy" medium (13C and 15N) and from bacteria grown in "light" medium (12C and 14N). These 70S ribosoms were added to an in vitro system engaged in protein synthesis. An aliquot removal serveral hours later was analyzed by density-gradient centrifugation. How many bands of 70S ribosoms would you expect to see in the density gradient? Is it possible to give a more comprehensive explanation? :)
The high rate of bacterial metabolism requires a high surface-to-volume ratio. Why does the surface/volume ratio affect the maximum metabolic rate? 2) Calculate the surface-to-volume ratio of the spherical Neisseria gonorhoe bacterium (0.5 µm in diameter). Compare this with the surface-to-volume ratio of the large eukaryote ameba (150 mm in diameter). 3) An enzyme isolated from rat liver has 193 amino acids and is encoded by a 1440 base pair long gene. Explain the connection between the amino acid number of the enzyme and the number of nucleotide pairs in its gene.
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The enzyme urease increases the rate of urea hydrolysis at pH 8.0 and 20 °C by a factor of 10^14. Suppose that a given quantity of urease can completely hydrolyze a given quantity of urea in 10 minutes at pH 8.0 and 20 °C. How long would it take for this amount of urea to be hydrolyzed in the absence of urease at the same temperature and pH in sterile conditions?
1. Doesn't non-fat milk protein contain casein which interacts with the Bradford reagent. why would this react with the Bradford or Bruiet reagent? 2. Tryptophan is an amino acid and there should not be a reaction with the reagent. Why would there be a reaction with the Bradford or Biuret reagent?
When Griffith incubated heat-killed virulent S strain bacteria with live avirulent R strains, he found that R cells were transformed into lethal, disease-causing bacteria. What is the molecule responsible for the transformation of R cells into the S type? Why did the transformation occur? A. Proteins in the heat-killed S cell extract; they were able to synthesize the capsular polysaccharide in the R cells and make them virulent. B. The capsular polysaccharide in the heat-killed S cell extract; the polysaccharide was able to attach to R cells, thus making them virulent. C. DNA in the heat-killed S cell extract; the DNA altered the genetic makeup of R cells, allowing them to synthesize the polysaccharide capsule, thus making them virulent. D. Specialized lipids in the cell membrane of the S cells were able to integrate with the R cells, conferring the ability to evade host immunity.
1. You have an F+ his- leu+ strain of E. Coli as well as a F- his+ leu- strain. Describe a strategy for how you might use these to isolate an Hfr strain.