1. Did water enter or leave the cells when the 5% salt solution was added? 2. What would you expect to happen if these cells were allowed to remain in the salt solution for several hours? 3. Imagine you pull away the cover slip and drip 10 drops of deionized water on the young Elodea leaf, rinsing away the 5% salt solution. Plasmolysis appears to reverse. Why is this happening?

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reached. Thus in plant cells, concentration is not the only force which influences water movement.
Pressure is also an important influence.
Plant cells are normally turgid as turgor pressure causes the cell membrane to be firmly pressed against
the cell wall, this provides a degree of rigidity for the cell. If the cell is placed in a hypertonic solution,
the solutes do not readily cross the cell membrane, causing water to diffuse out of the cell. The loss of
water reduces the volume of the protoplasm (everything from the cell membrane to inside the cell)
causing the cell membrane to pull away from the cell wall. This phenomenon plasmolysis.
View the demonstration: Plasmolysis in Elodea
https://www.youtube.com/watch?v=c8nhJBw S08
Procedure in the Demonstration
Imagine you are preparing a wet mount of a young Elodea leaf. You use 3 drops of 5% NaCl to wet the
slide with the young Elodea leaf and then place on the cover slip. Watch now what happens in the
demonstration (video above).
Questions
1. Did water enter or leave the cells when the 5% salt solution was added?
2. What would you expect to happen if these cells were allowed to remain in the salt solution for
several hours?
3. Imagine you pull away the cover slip and drip 10 drops of deionized water on the young Elodea
leaf, rinsing away the 5% salt solution. Plasmolysis appears to reverse. Why is this happening?
4. Plants do not have a skeleton. How can you account for the upright posture of plants?
5. Under what conditions do plants wilt? Explain the phenomenon using your knowledge of
osmosis. Include the words hypotonic, hypertonic, and isotonic in your explanation.
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Focus
W
P
T
✔
D
ENG
Transcribed Image Text:reached. Thus in plant cells, concentration is not the only force which influences water movement. Pressure is also an important influence. Plant cells are normally turgid as turgor pressure causes the cell membrane to be firmly pressed against the cell wall, this provides a degree of rigidity for the cell. If the cell is placed in a hypertonic solution, the solutes do not readily cross the cell membrane, causing water to diffuse out of the cell. The loss of water reduces the volume of the protoplasm (everything from the cell membrane to inside the cell) causing the cell membrane to pull away from the cell wall. This phenomenon plasmolysis. View the demonstration: Plasmolysis in Elodea https://www.youtube.com/watch?v=c8nhJBw S08 Procedure in the Demonstration Imagine you are preparing a wet mount of a young Elodea leaf. You use 3 drops of 5% NaCl to wet the slide with the young Elodea leaf and then place on the cover slip. Watch now what happens in the demonstration (video above). Questions 1. Did water enter or leave the cells when the 5% salt solution was added? 2. What would you expect to happen if these cells were allowed to remain in the salt solution for several hours? 3. Imagine you pull away the cover slip and drip 10 drops of deionized water on the young Elodea leaf, rinsing away the 5% salt solution. Plasmolysis appears to reverse. Why is this happening? 4. Plants do not have a skeleton. How can you account for the upright posture of plants? 5. Under what conditions do plants wilt? Explain the phenomenon using your knowledge of osmosis. Include the words hypotonic, hypertonic, and isotonic in your explanation. We're starting the add-ins runtime, just a moment... Focus W P T ✔ D ENG
Questions
1. If the potato cells were hypotonic to the surrounding solution, the surrounding solution itself
would be what to the potato cells (hypertonic, hypotonic, or isotonic)?
2. Were the potato cells submerged in water with no salt hypertonic, hypotonic, or isotonic to the
surrounding environment?
3. Were the potato cells submerged in 5 g/100mL salt solution hypertonic, hypotonic, or isotonic to
the surrounding environment?
4.
Look at your graph. At approximately what concentration of salt solution would the potato cells
have likely been isotonic with the surrounding solution? [Tip: Estimate approximately at what
concentration there would have been no change in the average length of potato strips.]
B. Turgor and Plasmolysis
You have observed that concentration differences between cells or between a cell and its environment
can affect the movement of water across cell membranes. This can be observed using changes in volume
or size as in the previous experiment or visually, looking at changes in appearance in the microscope, as
you will do as you explore this experiment.
The cells of plants are surrounded by a slightly elastic cellulose cell wall. If a plant cell is placed in a
hypotonic solution (the cell has a greater solute concentration than the surrounding medium), water will
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Transcribed Image Text:Questions 1. If the potato cells were hypotonic to the surrounding solution, the surrounding solution itself would be what to the potato cells (hypertonic, hypotonic, or isotonic)? 2. Were the potato cells submerged in water with no salt hypertonic, hypotonic, or isotonic to the surrounding environment? 3. Were the potato cells submerged in 5 g/100mL salt solution hypertonic, hypotonic, or isotonic to the surrounding environment? 4. Look at your graph. At approximately what concentration of salt solution would the potato cells have likely been isotonic with the surrounding solution? [Tip: Estimate approximately at what concentration there would have been no change in the average length of potato strips.] B. Turgor and Plasmolysis You have observed that concentration differences between cells or between a cell and its environment can affect the movement of water across cell membranes. This can be observed using changes in volume or size as in the previous experiment or visually, looking at changes in appearance in the microscope, as you will do as you explore this experiment. The cells of plants are surrounded by a slightly elastic cellulose cell wall. If a plant cell is placed in a hypotonic solution (the cell has a greater solute concentration than the surrounding medium), water will nglish (United States) Text Predictions: On Accessibility: Investigate O " a
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