Homework Help is Here – Start Your Trial Now!
Science
Biology
Positive sample negative sample 5. Unbound labeled antibody is washed away and a colorimetric substrate is added. Draw this Positive sample negative sample 6. The enzyme cleaves the substrate causing a color change of the substrate solution. The intensity the color is quantified using a spectrophotometer and is proportional to the amount of antibody present in the sample. (Obviously, a standard curve would have to be generated. Could you think how to do that?). Positive sample negative sample

Positive sample negative sample 5. Unbound labeled antibody is washed away and a colorimetric substrate is added. Draw this Positive sample negative sample 6. The enzyme cleaves the substrate causing a color change of the substrate solution. The intensity the color is quantified using a spectrophotometer and is proportional to the amount of antibody present in the sample. (Obviously, a standard curve would have to be generated. Could you think how to do that?). Positive sample negative sample

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
100%

Can anyone do second part?

Positive sample negative sample 5. Unbound labeled antibody is washed away and a colorimetric substrate is added. Draw this below Positive sample negative sample 6. The enzyme cleaves the substrate causing a color change of the substrate solution. The intensity of the color is quantified using a spectrophotometer and is proportional to the amount of antibody how to do that?). present in the sample. (Obviously, a standard curve would have to be generated. Could you think of Positive sample negative sample
Transcribed Image Text:Positive sample negative sample 5. Unbound labeled antibody is washed away and a colorimetric substrate is added. Draw this below Positive sample negative sample 6. The enzyme cleaves the substrate causing a color change of the substrate solution. The intensity of the color is quantified using a spectrophotometer and is proportional to the amount of antibody how to do that?). present in the sample. (Obviously, a standard curve would have to be generated. Could you think of Positive sample negative sample
The purpose of ELISAS is to detect specific protein antigens using antibodies. In laboratory medicine, ELISAS are commonly used to screen serum samples from animals to detect antibodies to a specific pathogen. In the research environment, ELISAS can be modified to detect a protein of interest. Method: 1. 96 well plates are coated with an antigen. Sites unoccupied by the antigen are blocked with a blocking buffer to prevent non-specific binding of sample. Draw this below: 2. Serum samples are added and incubated to allow specific antibodies to bind to specific antigen. Antibodies that do not recognize and bind specifically to the antigen remain unbound. Draw this below: Positive sample Negative sample 3. Non-specific antibodies that do not bind to the antigen are washed away. Draw this below. Positive sample Negative sample 4. An enzyme labeled (e.g. alkaline phosphatase or horseradish peroxidase) secondary antibody is incubated with the samples and binds tightly to sample antibody. Draw this below.
Transcribed Image Text:The purpose of ELISAS is to detect specific protein antigens using antibodies. In laboratory medicine, ELISAS are commonly used to screen serum samples from animals to detect antibodies to a specific pathogen. In the research environment, ELISAS can be modified to detect a protein of interest. Method: 1. 96 well plates are coated with an antigen. Sites unoccupied by the antigen are blocked with a blocking buffer to prevent non-specific binding of sample. Draw this below: 2. Serum samples are added and incubated to allow specific antibodies to bind to specific antigen. Antibodies that do not recognize and bind specifically to the antigen remain unbound. Draw this below: Positive sample Negative sample 3. Non-specific antibodies that do not bind to the antigen are washed away. Draw this below. Positive sample Negative sample 4. An enzyme labeled (e.g. alkaline phosphatase or horseradish peroxidase) secondary antibody is incubated with the samples and binds tightly to sample antibody. Draw this below.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 3 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Follow-up Questions
Read through expert solutions to related follow-up questions below.
Follow-up Question

Can you do the first part?

Solution
Bartleby Expert
SEE SOLUTION
Follow-up Question
Method: 1. 96 well plates are coated with an antigen. Sites unoccupied by the antigen are blocked with a blocking buffer to prevent non-specific binding of sample. Draw this below: 2. Serum samples are added and incubated to allow specific antibodies to bind to specific antigen. Antibodies that do not recognize and bind specifically to the antigen remain unbound. Draw this below: Positive sample Negative sample 3. Non-specific antibodies that do not bind to the antigen are washed away. Draw this below. Positive sample Negative sample 4. An enzyme labeled (e.g. alkaline phosphatase or horseradish peroxidase) secondary antibody is incubated with the samples and binds tightly to sample antibody. Draw this below.
Transcribed Image Text:Method: 1. 96 well plates are coated with an antigen. Sites unoccupied by the antigen are blocked with a blocking buffer to prevent non-specific binding of sample. Draw this below: 2. Serum samples are added and incubated to allow specific antibodies to bind to specific antigen. Antibodies that do not recognize and bind specifically to the antigen remain unbound. Draw this below: Positive sample Negative sample 3. Non-specific antibodies that do not bind to the antigen are washed away. Draw this below. Positive sample Negative sample 4. An enzyme labeled (e.g. alkaline phosphatase or horseradish peroxidase) secondary antibody is incubated with the samples and binds tightly to sample antibody. Draw this below.
Solution
Bartleby Expert
SEE SOLUTION
Knowledge Booster
Learning & Memory
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS