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- Question 1. How cell separation is key in cell culture establishment? Question 2. What is the relationship of adherent cells and trypsin in Animal cell culture? Question 3. An enriched medium containing salts, glucose, proteins and vitamins was made and a commercially available animal cell line was introduced. However, the cells began dying. What could be the reason behind it? Question 4. A) What is the significance of producing monoclonal antibodies? B) What is the role of cell culture in production of monoclonal antibodies? C) Name and briefly explain the use of any 4 commercially available monoclonal Question 5. The aim of a research project is to produce a hybrid plant having resistance to disease. The disease…Question 16 Within the context of infectious disease, which of the following specimens would be considered irretrievable? Question 16 options: a) Wound swab b) Blood sample for therapeutic drug monitoring c) Cerebrospinal fluid d) Urine sampleQuestion 5 You spread a small amount of a water sample from the environment on an enriched media agar plate. After 24 hours, the plate is covered with many small yellow colonies. Does this plate contain a pure culture of bacteria? ○ No, because it came from the environment, there are lots of different organisms. ○ We know there are multiple species present, but each colony is a different species. ○ Yes, we know the plate contains a pure culture because there are individual colonies. ○ We do not know if the plate contains a pure culture or a mixed culture. or even if individual colonies are pure or mixed based on their appearance alone.
- Question 24 Which of the following specimens should be kept at room temperature to maintain the growth and viability of the causative pathogen? Question 24 options: a) Urine and blood samples should always be kept at room temperature b) Blood and cerebrospinal fluid for bacterial cultures should always be kept at room temperature c) Blood and fecal specimens should always be kept at room temperature d) Urine and sputum samples should always be kept at room temperatureQuestion 7 Microbiology BIOL 2420 What does the line at the end of the arrow represent? Question 7 options: a) therapeutic index of the antibiotic b) minimum inhibitory concentration c) end of diffusion of the antibiotic d) more than one of theseQuestion 6 Which of the following is the correct sequence for the aseptic transfer of a bacterial culture? - 1. 2. 3. 4. 5. 6. 7. Remove the lid of the media receiving the culture & flame the mouth of this tube. - 1. 2. 3. 4. 5. 6. 7. Flame the mouth of the culture tube. - 1. 2. 3. 4. 5. 6. 7. Insert the wire loop with the culture to inoculate the bacteria on or in the new media. - 1. 2. 3. 4. 5. 6. 7. Reheat the mouth of the culture tube and replace the cap. - 1. 2. 3. 4. 5. 6. 7. Sterilize the loop by heating it from base to tip uniformly orange-hot. - 1. 2. 3. 4.…
- Question 15 Which of the following agents used for routine hand hygiene in health care settings is most bactericidal and least irritating to the skin? Question 15 options: a) Alcohol-based handrub b) Chlorhexidine handwash c) Plain soap and water d) Antimicrobial soap and waterquestion 11 Explain the role of streptomycin in the lab. 1. Streptomycin was the bacteria to which we applied the antibiotic 2. Streptomycin refers to the spreading process that precedes incubation. 3. Streptomycin is the growth medium that bacteria require to grow. 4. Streptomycin was the antibiotic to which we exposed the bacteriaQuestion 7 On which of these plates would you be most surprised to see colonies of E. coli growing after incubation? 1. antibiotic resistant E. coli on streptomycin positive plate 2. antibiotic resistant E. coli on streptomycin negative plate 3. antibiotic non-resistant E. coli on streptomycin positive plate 4. antibiotic non-resistant E. coli on streptomycin negative plate
- Question 1 Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You’re concerned that it’s an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium or a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. What is one experiment you could do, involving culturing the organism? Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we have done/could do in our lab. What is a procedure you could do, involving making a slide of the organism? Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we have done/could do in our lab. Without testing anything, how do you know this is not a viral infection?Question 8 How did we determine the degree of bacterial growth? 1. We approximated the number of colonies 2. we compared the weight of the plates before and after incubation 3. we watched for a color change in the agar 4. we watched for the release of metabolic gasesQuestion 5  Antibiotic resistance might be assumed if 1. bacterial colonies are present on a streptomycin negative plate 2. bacterial colonies are present on a streptomycin positive plate 3. bacterial colonies are absent on a streptomycin negative plate 4. bacterial colonies are absent on a streptomycin positive plate