1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog saline and the filled sack is placed in a beaker filled with the same saline. The recording electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the bath. a. (Draw a picture to help you visualize this arrangement.) b. When the electrodes are first placed, there is no transepithelial potential difference. c. After an hour, the transepithelial potential is +20, inside the sack relative to outside.

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter48: Regulating The Internal Environment
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1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog
skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the
internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog
saline and the filled sack is placed in a beaker filled with the same saline. The recording
electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the
bath.
a. (Draw a picture to help you visualize this arrangement.)
b. When the electrodes are first placed, there is no transepithelial potential
difference.
c. After an hour, the transepithelial potential is +20, inside the sack relative to
outside.
Transcribed Image Text:1. Frog skin is an excellent system for studying sodium transport across an epithelium. Frog skins is stripped from the frog's leg and tied into a sack. The inside of the sack is the internal (extracellular fluid) surface of the frog skin. The sack is filled with normal frog saline and the filled sack is placed in a beaker filled with the same saline. The recording electrode of a voltmeter is placed inside the sack and the ground electrode is placed in the bath. a. (Draw a picture to help you visualize this arrangement.) b. When the electrodes are first placed, there is no transepithelial potential difference. c. After an hour, the transepithelial potential is +20, inside the sack relative to outside.
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