1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock? 2. How do you prepare 50 mL of a 0.25X solution of Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?
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1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock?
2. How do you prepare 50 mL of a 0.25X solution of
Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?
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- How will you prepare a 5% BSA solution? Show all your steps.2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750 (ug/ml) E 500 (ug/ml) F 250 (ug/ml) G 125 (ug/ml) H 0 (ug/ml)How much of a 10x buffer should be added to 900ml of water to make a 1x solution?
- How would you prepare 3L of a basic buffer having a pH of 8.6 where the concentration of base is 0.1N?Which of the following is the best explanation for a gel like this?A. Bromophenol blue was not added in the loading buffer.B. Glycine was not present in the buffer system.C. There was too much bisacrylamide in the gel solution.D. Gel was not overlaid after pouring the first gel solution.You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many mL of D50W and sterile water are needed to complete the order? *
- I have: DMEM:F12 800X ITS 1.07 g/ml Linoleic acid Nu-serum (pure solution) BSA (pure powder) How much of each would I need to make 500ml of (1x ITS, 7.49 ug/ml linoleic acid, 4% v/v Nu-serum, 0.15% W/V BSA, and DMEM:F12 as the solvent)?I want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?