(Vmax.f [S] - (Vmax,b [P] KM,b [P] vo [S] 1+ KM.f KM.b ||
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In enzyme kinetics, for the reversible with two central complexes mechanism, please provide complete proof that the rate equation is the equation below. The variables denoted with f indicate forward direction while the variables denoted with b indicate backward direction.
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- How would cooler temperatures affect Km and Vmax on Michaelis-Menton curve?Using the Michaelis-Mentan graph pictured (graphing initial velocity and PNPP concentration), identify Vmax and Km as well as possible. Then, using the Lineweaver-Burk graph (graphing 1/Vo and 1 / PNPP concentration), label the reciprocals of Vmax and Km on the graph and use them to calculate Km and Vmax.Calculate the Vmax with the parameters given. All parameters may not be needed to calculate. Kcat = 130 s^-1 Vo = 3.0 μMs^-1 S = 10 μM Et = 0.15 µM
- Using the above “mean-speed theorem”, calculate the average velocity of a car with constant acceleration from 0 km/hour (initial speed) to 80 km/hour (final speed) over a 5-minute period of time. average velocity over 5 minutes = 16 kilometers/hour average velocity over 5 minutes = 20 kilometers/hour average velocity over 5 minutes = 24 kilometers/hour average velocity over 5 minutes = 32 kilometers/hour average velocity over 5 minutes = 40 kilometers/hourA balloon occupies a volume of 1.25 L at sea level where the ambient pressure is 1 atm. What volume would the balloon occupy at an altitude of 10,700 m, where the air pressure is only 220 mmHg?The “mean-speed theorem” for calculating average velocity under constant acceleration, developed by Thomas Bradwardine and the Mertonian Calculators at Oxford University, is expressed algebraically as: density = weight/volume (m1)(v1) = (m2)(v2) C. (vm) = 1/2 (v0 + vf) s = (v0)(t) + 1/2 (a)(t2) velocity = distance/time