1. To determine the sequence of amino acids in protein, partial hydrolyses at different points are made and then compared to other sets of partial hydrolyses For example: Complete hydrolysis gave: Phenylalanine-1 Histidine-2 Glutamic acid-1 Leucine - 4 Asparagine-1 Cystine -2 Glutamine-1 Glycine - 2 The Partial Hydrolyses yielded the following: 1st Hydrolysis : 4 polypeptides ● . IA: phe-val-asn-gln-his-leu IB: IC: leu-tyr-leu-val ID: val-cys-gly 2nd Hydrolysis: 3 polypeptides IIA: phe-val-asn-gIn-his-leu-cys-gly-ser-his-leu IIB: val-glu-ala-leu-tyr-leu IIC: val-cys-gly Write down the primary structure of this polypeptide by finding the overlaps of the amino acids therein: Serine-1 Valine-3 Alanine-1 tyrosine-1 cys-gly-ser-his-leu-val-glu-ala

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. To determine the sequence of amino acids in protein, partial hydrolyses at different points
are made and then compared to other sets of partial hydrolyses For example: Complete
hydrolysis gave:
Phenylalanine-1
Histidine-2
Glutamic acid-1
Leucine - 4
Asparagine-1
Cystine -2
Glutamine-1
Glycine - 2
The Partial Hydrolyses yielded the following:
1st Hydrolysis : 4 polypeptides
●
.
IA: phe-val-asn-gln-his-leu
IB:
IC: leu-tyr-leu-val
ID: val-cys-gly
2nd Hydrolysis: 3 polypeptides
IIA: phe-val-asn-gIn-his-leu-cys-gly-ser-his-leu
IIB: val-glu-ala-leu-tyr-leu
IIC: val-cys-gly
Write down the primary structure of this polypeptide by finding the overlaps of the amino acids
therein:
Serine-1
Valine-3
Alanine-1
tyrosine-1
cys-gly-ser-his-leu-val-glu-ala
Transcribed Image Text:1. To determine the sequence of amino acids in protein, partial hydrolyses at different points are made and then compared to other sets of partial hydrolyses For example: Complete hydrolysis gave: Phenylalanine-1 Histidine-2 Glutamic acid-1 Leucine - 4 Asparagine-1 Cystine -2 Glutamine-1 Glycine - 2 The Partial Hydrolyses yielded the following: 1st Hydrolysis : 4 polypeptides ● . IA: phe-val-asn-gln-his-leu IB: IC: leu-tyr-leu-val ID: val-cys-gly 2nd Hydrolysis: 3 polypeptides IIA: phe-val-asn-gIn-his-leu-cys-gly-ser-his-leu IIB: val-glu-ala-leu-tyr-leu IIC: val-cys-gly Write down the primary structure of this polypeptide by finding the overlaps of the amino acids therein: Serine-1 Valine-3 Alanine-1 tyrosine-1 cys-gly-ser-his-leu-val-glu-ala
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