You are given a pure protein sample to characterize and provided the following information: Its molar extinction coefficient, ε280, is 0.25 liters micromole^-1 cm^-1 Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 20- fold dilution of your pure protein in solution (by this, we mean that 50 ul of the protein sample was diluted to a final volume of 1 ml) and find A280 = 0.40. What is the original concentration of the protein before dilution?

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Physical and Chemical features

The physical property of a compound could be determined without modifying the originality of the compound. Examples of physical properties are density, hardness, color, boiling, and melting point. The chemical property expresses the ability of a compound to undergo a particular chemical change.