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- What are hyphenated GC methods? Briefly describe two hyphenated methods.PART1: Put True (T) or False (F) for the following sentences: 1. chromatography" derived from two Greek words "Chroma" meaning color and "graphein" meaning to write. The stationary phase may be a solid, or a liquid supported on a solid or gel. 3. The mobile phase may be 2. Solid. 4. Silica gel is the most common stationary phase in adsorption chromatography. 5. Liquid Chromatography abbreviated as (GC ). 6. In Thin Layer Chromatography, the mobile phase is gas. 7. a = KB/KA, where Kg is the distribution constant. 8. k'B and k'A are the retention factors. 9. Rf, is the distance traveled by the compound ONLY. 10. Chromatography is a physical method of separation.1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.
- CHROMATOGRAPHY HW 1. What does adsorb mean? 2. What is chromatography used for? 3. Why must a pencil rather than a pen be used to mark the TLC plate? 4.If you make an error recording data, how would you correct it? Is it ok to erase it or white it out? 5.Using the data on the last slide, calculate the Rf value for each spot.1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?Name 3 adsorbents used in TLC. Mention their use and properties. Why is it important to use a pencil, and not pen, for marking TLC plates?
- Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?19. What is thin layer chromatography (TLC) LEAST useful for from the list below? A) To determine if crystallization or distillation may be more effective for a separation B) To determine the number of components in a mixture C) To determine the appropriate solvent for column- chromatographic separation D) To monitor the progress of an organic synthesis reaction 14. The solubility of salicylic acid in water is 7.8g/100ml at 100°C and 0.25g/100ml at 25°. Estimate how much water is needed to recrystallize a 19g sample of salicylic acid? A) 150ml B) 250ml C) 300ml D) 400mlWhat is the stationary phase in thin layer chromatography and its polarity? Choices: Cellulose - non-polar Cellulose - polar Silica - non-polar Silica - polar
- The uses of thin layer chromatography are: 1) To identify unknown compounds 2) Determine purity of products 3) Analyze reaction mixtures 4) To waste time in lab 5) Determine the melting point of the product U 6) Put an astronaut on the moon.what is chromatography? Explain different types of chromatography and explain how to seperate compounds. indicate stationary phase mobile phase.In thin-layer chromatography on silica gel TLC plates, which of these is true? a. More polar compounds move slower because they have greater affinity for the polar silica. b. More polar compounds move slower because they have lesser affinity for the polar silica. c. More polar compounds move faster because they have lesser affinity for the polar silica. d. Less polar compounds move slower because they have lesser affinity for the polar silica.