1. Which of these methods will allow cell counting and segregation? a. Flow Cytometry b. Western Blot c. Immunocytochemistry d. ELISA 2. Which of the following is not a consequence of a CRISPR/Cas9-mediated double strand break that occurred in the absence of a homologous chromosome? a.The target sequence will be repaired without errors b.The target sequence will be repaired with errors c.The target sequence will incur deletions d.The target sequence will incur insertions.

1. Which of these methods will allow cell counting and segregation? a. Flow Cytometry b. Western Blot c. Immunocytochemistry d. ELISA 2. Which of the following is not a consequence of a CRISPR/Cas9-mediated double strand break that occurred in the absence of a homologous chromosome? a.The target sequence will be repaired without errors b.The target sequence will be repaired with errors c.The target sequence will incur deletions d.The target sequence will incur insertions.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1TYK

See similar textbooks

Similar questions

1. Which of these methods will allow quantification of a vaccine? a. Immunocytochemistry b. Flow Cytometry c. Western Blot d. ELISA 2. Sanger sequencing depends on _____________ during in vitro DNA replication a.random incorporation of fluorescent-labelled deoxynucleotides which releases a color after being added to the DNA chain b.random incorporation of pyrophosphate-labelled deoxynucleotides which a releases color upon addition of substrate c.random incorporation of fluorescent-labelled dideoxynucleotides which terminates the growing chain d. subsequent ligation of a DNA octamer with two known specific bases
Antibody diversity is produced bya. V(D)J recombination.b. imprecise end joining during V(D)J recombination.c. somatic hypermutation.d. all of the above.
11 Enzyme assay A) Always involves asking a substrate B) Can be very smoke or very complex C) Always involves ultraviolet light D) A-C are incorrect 12 Isolation of chromosomal (total) DNA using a spin column A) Involves a filtration step to remove many different molecules B) Lysis is an optional step C) Elution is not necessary D) Results in a very pure protein preparation 13 Isolation of chromosomal (total) DNA using a spin column A) Cells must be used using an appropriate method B) Cell lysis is required C) Elution of DNA required regardless of the cell type used in the experiment D) A-C are correct. 14 Isolation of chromosomal (total) DNA using a spin column A) Washing the spin column is optional B) DNA must be eluted from the spin column C) Does not involve centrifugation D) Only molecules that are supposed to bind to the column do so.
1. Write if the statement is true and modify the statement with the correct answer if its false a. E. coli strains used in blue-white screening produce blue color complexes because their beta-galactosidases are activated via alpha-complementation. b. If you are a researcher trying to identify a gene that is responsible for a trait of interest, the first thing that you would do is to make a review of the literature that is available. c. The polylinker region in plasmids is where specific DNA sequences are cut by non-specific restriction enzymes. d. Blue-white screening is one of the last steps involved in determining if the gene of interest has been inserted successfully in plasmids.
During Western blotting, the primary antibody recognizesa. the secondary antibody.b. the protein of interest.c. an mRNA of interest.d. a specific fragment of chromosomal DNA.
describe each step carefully and use your own words. Steps will be like following; I) Primer design for SLIC cloning and the protocol II) Transformation and sequence validation of the clones III) Culture of HEK293T IV) Transduction of HEK293T cells with plasmid V) Western Blotting VI) Immunfluorescence for Confocal Microscopy
In step 2 (above) in the identification of the colony that contains a targeted gene, ____. a. bacterial cells are cloned b. cells with the targeted gene are identified c. bacterial colonies are transferred d. DNA is lysed e. bacterial cells are ruptured
Which nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.
One of the following statements is FALSE A. Labeled antibodies are the tagged probes of Southern blotting and ELISA (enzyme linked immunosorbent assay) B. In biopharmaceutical engineering; immunogenicity and pharmacokinetic properties are altered favorably; hence therapeutically C. In RFLP (restriction fragment length polymorphism), number and length of restrictionfragments vary due to genome polymorphism and bases sequencevariations D. In gene replacement therapy a normal gene allele substitutes permanently a mutant allele in a somatic cell
(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.
Which one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0
You have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligation