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- If you make a five-tube twofold dilution using 2 mL of serum, what is the concentration of serum in tube #4?Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μLComplete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9