12. What transport rates for each condition would you expect if the experiment was repeated using 12µM [³H] serotonin? Add your estimate into the table above. 13. Examine the plot obtained for condition A; what can you conclude with regards to whether or not the transport of serotonin into these cells requires a protein? Explain your conclusion.
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- Penicillin heralded the dawn of the antibiotic age when it was discovered in 1928 and is now the most widely used antibiotic in the world. A membrane electrode was developed for the quantitative analysis of penicillin. The table above shows the data which were collected using a set of penicillin standards against saturated calomel electrode. An unknown sample produced a potential of 142 mV. A) Which served as the reference electrode? B) What is the molar concentration of penicillin in the sample?Salt Concentration (%) Transmittance (%) 0 77.23 3 79.23 6 80.39 9 82.71 12 85.66 15 87.55 What is the dependent variable and the independent variable? what patterns do you observe based on the information in the table?Using two centrifuges (A and B), with maximumspeeds of 1500 rpm (r = 8 cm) and 3000 rpm(r = 8 cm), respectively, construct a separationscheme for separating three subcellular structureswith the following xg requirements:• PAT – 150 xg• SAT – 120,000 xg• HAT – 650 xg
- Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computationsIn an electrochemical cell, Q = 0.00025 and K = 0.70. What can you conclude about Ecell and E°cell? Ecell is positive and E°cell is positive Ecell is negative and E°cell is positive Ecell is negative and E°cell is negative Ecell is positive and E°cell is negativeYou have two solutions that are isosmotic to each other and to a cell. However, when testing for tonicity, one of these solutions is hypotonic and one is isotonic. Briefly explain how this is possible.
- Twenty observations on etch uniformity on silicon wafers are taken during a qualification experiment for a plasma etcher. The data are as follows: Etch Uniformity 5.34 6.65 4.76 5.98 7.25 6.00 7.55 5.54 5.62 6.21 5.97 7.35 5.44 4.39 4.98 5.25 6.35 4.61 6.00 5.32 (a) Discuss the normality assumption and its role in this problem. (b) Check normality by constructing a normal probability plot. What are your conclusions?Which of the phi/psi angles would be violating a ramachandran plot? Select all that apply: Phi= 90; Psi=-120 Phi= -120; Psi=120 Phi= 0; Psi=0 Phi= -90; Psi=-60 Phi= 120; Psi=-120Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?
- Non-specific interactions present a real challenge to co-IP experiments because non-physiological binding to the target complex is likely to occur. Group of answer choices True FalseFrom your results, which component had the highest Rf value and which had the lowest? Based on your answer from the previous question, what could be the reason behind some components having an Rf value higher than the others? Compare the qualities of the two components that rendered the highest and lowest Rf After performing the simulation, what do you think are the possible practical applications of chromatographic techniques that are relevant to your program?Hello, I still can't understand why we get about 24500 signals. Please let me know what formula I should use.