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- 1. a)how is it possible for such drugs to selectively kill bacterial cells and not our own cells? b)Provide an example of post-translational regulation of protein activity and explain the advantage of regulating each protein/process at the post-translational level instead of the transcriptional level.62) If you want to inhibit protein synthesis on the transcriptional level you would use: a) Tetracyclin b) Vancomycin c) Macrolides d) Fluroquinolones e) a & c 63) The protein that prevents the formation of off-centered septation in bacteria is: a) FtsZ b) MreB c) MinCD d) Tubulin e) Septin 64) The autoclave pressure must reach at least ________ in order to effectively kill the bacterial spores: a)15 psi b) 15 Joules c) 15 Kilograms d) 15 Lbs e) 15 Kilocalories 65) Incineration is a type of ______ method in controlling bacterial growth: a) moist heat b) dry heat c) chemical d) chemotherapeutic e) genetic.Explain why RNase A cannot catalyze the hydrolysis of DNA.
- Why can it be said that transcription of thebacteriophage T7 genome requires two enzymes?38. Assume that the reading frame is from left to right and that the columns represent transcriptional and translational alignments. Use the provided Genetic Code table.A normal mRNA that reads 5’ - UGCCAUGGUAAUAACACAUGAGGCCUGAAC- 3’ has an insertion mutation that changes the sequence to 5' -UGCCAUGGUUAAUAACACAUGAGGCCUGAAC- 3’. Translate the original mRNA and the mutated mRNA, and explain how insertion mutations can have dramatic effects on proteins. (Hint: Be sure to find the initiation site.)
- A scientist discovers a virus encoding a Protein X that degrades a subunit of the elF4F complex. Knowing that this virus transcribes its own mRNAs in the cytoplasm of human cells, why would Protein X be an effective virulence factor?A fragment of bacterial DNA reads: 3’ -TACCTATAATCTCAATTGATAGAAGCACTCTAC- 5’ Assuming that this fragment is the template strand, what is the sequence of mRNA that would he transcribed? (Hint: Be sure to identify the initiation site.)A scientist identifies a potential transcription regulation site 300bp downstream of a gene and hypothesizes that it is a repressor. What experiment (with results) could he perform to support this hypothesis?
- Quinolone antibiotics treat bacterial infections by blocking the activity of topoisomerase. Why does this treatment work? Explain what occurs at the molecular level.Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.In prokaryotes, _________bind to a sequence called theShine-Dalgarno box adjacent to the AUG initiation codon.?
