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- Detail the differences between base excision repair and nucleotide excision repair. Please help explain in 5 sentences or less, thank you!PLEASE HELP ME ANSWER THESE OBJECTIVES QUESTIONS. THANK YOU IN ADVANCE. 1. In genetic engineering, a reverse transcriptase enzyme is used to a. replace the Pfu polymerase enzyme b. denature all messenger RNAs c. undo the action of a restriction enzyme d. produce complementary DNA 2. In lac operon, both gene A and gene B undergo a transcription process. Gene B can only undergo transcription in the presence of lactose and in the absence of glucose. The product of gene A is often altered by an inducer. Which of the following is true about genes A and B? a. Gene A= structural gene; Gene B= regulatory gene b. Gene A= promoter gene; Gene B= operator gene c. Gene A= lacZ gene; Gene B= promoter gene d. Gene A= regulatory gene; Gene B= structural gene 3. In response to a newly encountered pathogen, the first antibody class to appear in the serum is ________. a. IgE b. IgM c. IgA d. IgG 4. Lymphocytes that involve in the defense system by means of direct attack…1.) Chronic Myelogenous Leukemia (CML)-Briefly describe the disease and provide the most common symptoms/features of the disease.-Describe the cause/s of the disease.-Using a diagram, outline the processes of the best DNA technology tools/techniques involved in the diagnosis of the disease.-Present the best therapeutic DNA technology tool/technique for the disease by describing the tool and discussing the procedure.-Identify the challenges or issues that a scientist may face for using the DNA technology diagnostic and therapeutic tools and applications you have presented.
- Please help me to answer the following: 1. Explain how the synthesis of a DNA daughter strand growing toward a replication fork differs from the synthesis of a daughter strand growing away from a replication fork. 2. The base sequence ACGTCT represents a portion of a single strand of DNA. Please draw the complete double stranded molecule for this portion of the strand and use the representation to illustrate the replication of the DNA strand.Please clearly identify the nucleotide bases involved, the new strands formed, and the daughter DNA molecules. 3. Please explain the origin of Okazaki fragments. Thank you very muxh for your help.Please help with sub questions 1a 1b and 1c DNA: 1a. Whats the difference between continuous and discontinuous synthesis? 1b. Why replication requires both? 1c. How was this determined or How was this discovered?12.please match with the type of mutation
- B. Using the DNA sequence above, write a new DNA sequence from 3’ to 5’ that incorporates a transition leading to a silent mutation in the second amino acid. Bold or underline the nucleotide that has been changedplease answer these questions for this image What is the source of the DNA? What is the target? What vector is being used to move the DNA? What is the benefit of transforming the target cell in these ways?Discuss the following mutations with reference to specific genetic disorders: i) Faulty DNA repair; ii) Gain-of-function; and iii) Trinucleotide repeats. Give steps for each mutations.
- Discuss some potential issues regarding gene editing to correct genetic defects.Discuss about conservative replication ?Please help with 2a) 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA PolymeraseIII, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. colistrain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-functional) - assuming that all the other enzymes involved in replication remained fullyfunctional, how would DNA replication in these mutant cells without DNA Pol III differ fromDNA replication in normal E. coli? Briefly explain why you would expect to see thatchange/those changes in DNA replication in the mutant cells.