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- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?Discuss the types of interferences in atomic absorption spectrophotometry.1. What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer?
- 2. When working with AAS, tell the criteria to work with which flame and in which part of the flame?3. Tell the variables which affect absorbance and fluorescense.?4. What are the light sources used in UV-vis spectrophotometry.?if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
- Besides 1H NMR, FT-IR, melting point, data for refractive index, what other information could you obtain for your compound to assess its’ purity and confirm its’ structure? How would this additional data contribute to your conclusion about the structure of the unknown compound?What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and dilution factors? What are 2 disadvantages?In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
- What is the main application of Atomic absorption spectrophotometer (AAS)?Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?1. after going through all 4 objectives, which would give you the largest field of view? greatest depth of focus? why? 2. how do you calculate the total magnification of a specimen? show sample