Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?
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Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?
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- Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.Why don’t most light microscopes use 303 ocular lenses for greater magnification?If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400x
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.Which of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?
- if 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved?If 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved? Explain your answer.Why did we calculate, rather than measure, the diameter of the field of view on medium and high power? When using the compound microscope, you always begin viewing your specimen on __________ power, and use the __________ focus knob.
