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Topic: Pour-Plate Method
2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚C
Effect:
Rationale:
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- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:Make illustrated diagrams of stepwise procedures for the performance of the following tasks. 2. Sterilization of liquid culture media for platingRead the following statements. Fill-in the possible effect of the procedure and give the rationale. (STERILITY TEST) The antimicrobial activity of the product tested was not eliminated. Effect: Rationale:
- Give the drug examples of emulsion prepare by these method:- 1)dry gum method 2)wet gum method 3)bottle gum methodTopic: Spread-Plate Method Agar was not completely set prior to inoculating with the organism. Effect:Rationale:Define the following: 1. Asepsis: 2. Disinfectant: 3. Antiseptic: 4. Sanitization: 5. Bacteriostatic: 6. Bactericidal: 7. Thermal death point (TDP): 8. Thermal death time (TDT): 9. Decimal Reduction Time (DRT):
- What appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobMake illustrated diagrams of stepwise procedure for the performance of the following tasks applying appropriate aseptic techniques. 1. Transfer of Broth Culture to Plated Solid MediaGive at least five different microbial control methods you have on your home as listed on the pre-task activity. Identify its microbial control method (Physical or chemical), Specific component (heat, phenol, etc), Mode of action (denatures protein, disrupt cell membrane, etc), Use (sterilize, disinfect, antiseptic), Level of activity (Sterilant, high, intermediate, low disinfectant) MICROBIAL CONTROL METHOD SPECIFIC COMPONENT MODE OF ACTION USE LEVEL OF ACTIVITY
- Make illustrated diagrams of stepwise procedure for the performance of the following tasks applying appropriate aseptic techniques. 2. Transfer of Broth Culture to Tubed Solid MediaDiscuss the procedure and application for the agar well diffusion method of evaluating antimicrobial activity.Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%