color and 6 being intense coloration (red). Test Tube # Treatment Color Intensity 1 1% Acetone 3 2 25% Acetone 5 3 50% Acetone 4 1% Methanol 4 3 5 25% Methanol 4 6 50% Methanol 6 Solvent Variant Beet Membrane Disruption 6.
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- Draw and label a cell lipid bilayer and diagram how the following transport processes take place: passive diffusion of oxygen into the cell, facilitated diffusion of potassium into the cell, active transport of sucrose into the cell. Using different symbols (circles for oxygen, squares for sucrose, and triangles for potassium ions), show the relative concentrations (gradients) of these substances on the inside and outside of the cell. For example, to show that oxygen enters the cell by going “down” its gradient, you would draw more circles on the outside of the cell than inside the cell. Be sure to show and label membrane proteins when appropriate, and show the electric membrane potential using “+”s (pluses) on one side and “-”s (minuses) on the other side of the membrane. Also show the proton pump which uses ATP as a source of energy.Secretory vesicles (destined for exocytosis) that fuse with the plasma membrane only in an intermittent manner (often requiring an environmental signal) are properly referred to as: constitutive secretory vesicles (like those that carry lipids, such as phosphatidylcholine) the v-SNARES the endosomes the t-SNARES regulated secretory vesicles (like those that carry hormones, such as insulin)While performing fluorescent microscopy to observe the transport of bulk molecules into a cell a researcher uses a green, fluorescent bulk protein and a red fluorescent dye to stain the cell membrane. If the cell has the ability to transport the bulk proteins into the cell, what would we expect to see in the microscope when the images are merged? What would we expect to see if the cell did not have the ability to transport the bulk protein?
- Which of the following is/are true about the Cellular Membrane Structure: A. All membranes from plants, animals and microorganisms have similar biomolecular ultrastructure but varies in the types of lipids found. B. All membranes examined that have 2 dark-staining layers in the electron microscope indicated the presence of a layer exposed to the outer cell surface and another facing the cytiplasmic layer. C. The plasma membrane of cells are seen as thin, rigid, gel-like structure that is around 5-10nm thick. D. Some membranes have more protein components and some have similar percentage composition as the lipids. A, B, C only B, C, D only A, C, D only A, B, D onlyList and describe the three main types of cytoskeleton. If you wanted to do immunocytochemistry to specifically stain each type of cytoskeleton, what is a protein that could be used for each cytoskeletal type (in other words, what is a unique protein for each cytoskeletal type)? What are three types of actin structures? Describe the involvement of actin structures in cell migration. How is the growth and shrinking of microtubules regulated? Then describe the roles of microtubules in: chromosomal separation during mitosis and the movement of organelles and vesicles within a cell. Describe a possible mechanism on how signaling pathways might impact the cytoskeleton, so that cell migration could be regulated in a localized manner within a multicellular organism. (hint: think about the possible transcriptional regulation of the G-protein regulators) What are 2 main challenges of protein targeting? Then describe one way these challenges are overcome during signal-based targeting and one way…After pinocytic vesicles fuse with early endosomes, one possible fate of both cargo receptors and their cargo could be translocation to a new plasma membrane location, followed by exocytosis of the cargo molecule, which commonly occurs in human gut epithelial cells carrying which cargo? the human immunodeficiency virus (HIV) the glucose molecule the low density lipoprotein (LDL) the influenza virus the cholesterol molecule
- An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane (but with no cell wall) is immersed in a beaker containing an aqueous solution. The outside environment concentration consists of 0.01 M glucose and the inside of the cell has a concentration of 5.0 M glucose. The plasma membrane is permeable to water and monosaccharides, but impermeable to the disaccharides. Complete the following for the image below: Will the artificial cell become lyse, shrivel, or stay the same? lyse shrivel stay the sameWhich of the following processes results in the formation of food vesicle within the cell whose contents will subsequently be digested by enzymes in the lysosome a) receptor mediated endocytosis b) phagocytosis c) pinocytosis d) exocytosisCell motility has been described as being like the motion of tank treads. At the leading edge, actin filaments form rapidly into bundles and networks that make protrusions and move the cell forward. At the rear, cell adhesions are broken and the tail end of the cell is brought forward. What provides the traction for moving cells? How does cell-body translocation happen? How are cell adhesions released as cells move forward?
- Which of the following statements best describes the synthesis of secreted proteins? a.) Secreted proteins are typically synthesized in the smooth endoplasmic reticulum and carried to the Golgi apparatus in vesicles. b.) Secreted proteins are typically synthesized in the rough endoplasmic reticulum and carried to the Golgi apparatus in vesicles. c.) Secreted proteins are typically synthesized in the Golgi apparatus. d.) Secreted proteins are typically synthesized in the cytoplasm and diffuse to the Golgi apparatus.An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane (but with no cell wall) is immersed in a beaker containing an aqueous solution. The outside environment concentration consists of 0.01 M glucose and the inside of the cell has a concentration of 5.0 M glucose. The plasma membrane is permeable to water and monosaccharides, but impermeable to the disaccharides. Complete the following for the image below: Is the solution inside the cell isotonic, hypotonic, or hypertonic? Is the glucose going down or against its concentration gradient? Draw solid arrow to indicate the movement of the solute into or out of the cell. Draw a dashed arrow to show the movement of water. Will the artificial cell become lyse, shrivel, or stay the same?In addition to providing support, the cytoskeleton immobilizes enzymes and moves organelles to suitable positions in the cytoplasm. What advantage do these circumstances have over allowing the cell contents to freely diffuse in the cytoplasm?