color and 6 being intense coloration (red). Test Tube # Treatment Color Intensity 1 1% Acetone 3 2 25% Acetone 5 3 50% Acetone 4 1% Methanol 4 3 5 25% Methanol 4 6 50% Methanol 6 Solvent Variant Beet Membrane Disruption 6.

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Chapter3: Cell Structure
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Need help with the temperature exposure questions
ORGANIC SOLVENT EXPOSURES: The color scale represents subjective assessments with 0 being no
color and 6 being intense coloration (red).
Test Tube # Treatment
Color Intensity
1 1% Acetone
3
2 25% Acetone
3 50% Acetone
4
4 1% Methanol
5 25% Methanol
6 50% Methanol
Solvent Variant Beet Membrane Disruption
6
IAcetone
IMethanol
1.00%
25.00%
50.00%
Percent Solution
2 |Page
Temperature Exposures: Analyze the Results
What temperatures were the most damaging?
Why are the temperatures that you selected the most damaging? i.e. How do you know there is
damage? Describe a possible mechanism that underlies the damage for each temperature selected.
What temperatures were the least damaging or not damaging at all? How can you tell?
Organic Solvent Exposures: Analyze the Results
Which organic solvents and at what percentages were the solvents the most damaging?
Why are those solvents you selected the most damaging? i.e. How do you know there is damage?
Describe a possible mechanism that underlies the damage for each solvent selected.
What might account for the difference in impact between the two solvents use in the experiment?
Transcribed Image Text:ORGANIC SOLVENT EXPOSURES: The color scale represents subjective assessments with 0 being no color and 6 being intense coloration (red). Test Tube # Treatment Color Intensity 1 1% Acetone 3 2 25% Acetone 3 50% Acetone 4 4 1% Methanol 5 25% Methanol 6 50% Methanol Solvent Variant Beet Membrane Disruption 6 IAcetone IMethanol 1.00% 25.00% 50.00% Percent Solution 2 |Page Temperature Exposures: Analyze the Results What temperatures were the most damaging? Why are the temperatures that you selected the most damaging? i.e. How do you know there is damage? Describe a possible mechanism that underlies the damage for each temperature selected. What temperatures were the least damaging or not damaging at all? How can you tell? Organic Solvent Exposures: Analyze the Results Which organic solvents and at what percentages were the solvents the most damaging? Why are those solvents you selected the most damaging? i.e. How do you know there is damage? Describe a possible mechanism that underlies the damage for each solvent selected. What might account for the difference in impact between the two solvents use in the experiment?
A) Membrane integrity is critical for cell structure and function. In the lab, a useful experimental tool
used to illustrate this is the beet root. See below for an image from the Old Farmer's Almanac of the
beet (Beta vulgaris), a root vegetable. Credit: Darasp Kran/Shutterstock.
Each beet cell has a large central vacuole that stores betacyanin, a reddish pigment. The membrane that
serves as the boundary for the vacuole is called the tonoplast and it, like the plasma membrane, is
sensitive to damage when exposed to varied environmental conditions. Credit: Slideserve.com.
Beetroot cell vacuoles
The results below were obtained after exposure of beet root cylinders to varied temperatures and
organic solvents. This was done to assess the impact of physical and chemical factors on the integrity of
the cellular membranes.
Results (From Pockat AP Biology)
TEMPERATURE EXPOSURES: Six different temperatures were used (°C) and the color scale represents
subjective assessments with 0 being no color and 5 being intense coloration (red).
Beets Samples (Color
Intensity)
Tube # Treatment
1
70
2
55
1
3
40
20
5
6
-5
5
Temperature Variant: Beet
Membrane Disruption
I Beets
I J
4.5
1.5
70
55
40
20
5 -5
Temperature Treated
Transcribed Image Text:A) Membrane integrity is critical for cell structure and function. In the lab, a useful experimental tool used to illustrate this is the beet root. See below for an image from the Old Farmer's Almanac of the beet (Beta vulgaris), a root vegetable. Credit: Darasp Kran/Shutterstock. Each beet cell has a large central vacuole that stores betacyanin, a reddish pigment. The membrane that serves as the boundary for the vacuole is called the tonoplast and it, like the plasma membrane, is sensitive to damage when exposed to varied environmental conditions. Credit: Slideserve.com. Beetroot cell vacuoles The results below were obtained after exposure of beet root cylinders to varied temperatures and organic solvents. This was done to assess the impact of physical and chemical factors on the integrity of the cellular membranes. Results (From Pockat AP Biology) TEMPERATURE EXPOSURES: Six different temperatures were used (°C) and the color scale represents subjective assessments with 0 being no color and 5 being intense coloration (red). Beets Samples (Color Intensity) Tube # Treatment 1 70 2 55 1 3 40 20 5 6 -5 5 Temperature Variant: Beet Membrane Disruption I Beets I J 4.5 1.5 70 55 40 20 5 -5 Temperature Treated
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