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Topic: Preparation of Microbial Suspension
5. Over-dilution with sterile water.
Effect:
Rationale:
Step by step
Solved in 3 steps
- Read the following statements. Fill-in the possible effect of the procedure and give the rationale. (STERILITY TEST) The antimicrobial activity of the product tested was not eliminated. Effect: Rationale:After a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE:Define the following: 1. Asepsis: 2. Disinfectant: 3. Antiseptic: 4. Sanitization: 5. Bacteriostatic: 6. Bactericidal: 7. Thermal death point (TDP): 8. Thermal death time (TDT): 9. Decimal Reduction Time (DRT):
- Give at least five different microbial control methods you have on your home as listed on the pre-task activity. Identify its microbial control method (Physical or chemical), Specific component (heat, phenol, etc), Mode of action (denatures protein, disrupt cell membrane, etc), Use (sterilize, disinfect, antiseptic), Level of activity (Sterilant, high, intermediate, low disinfectant) MICROBIAL CONTROL METHOD SPECIFIC COMPONENT MODE OF ACTION USE LEVEL OF ACTIVITYTopic: Preparation of Microbial Suspension 1. Working in a poorly lit laboratory. Effect: Rationale:Discuss the procedure and application for the agar well diffusion method of evaluating antimicrobial activity.
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:list 5 different antimicrobial agents, describe their mode of action and whether the are an HLD, ILD or LLD.Antimicrobial agent Disk code zone of inhibition interpretation Ampicillin AM carbenicillin erythromycin gentamycin Nalidixic acid