Caculate the concentration of DNA of each tube (1-8) at the end, including the unkown. The DNA concentration is 1.5 C. Estimating DNA concentration - creating a 2-fold dilution series.Can you estimate how much DNA is an unknown sample?1. Label an 8-tube strip of PCR tubes• Label the first seven tubes 1-7. Label the final tube "U" for unknown.2. To tube 1, add 10 µl of 50:50 AT:GC DNA (tube B).3. Add 5 µl of Buffer to tubes 2-7.4. Remove 5 µl from tube 1 and add it to tube 2.• Pipette up and down gently three times to mix.5. Remove 5 ul from tube 2 and add it to tube 3.• Pipette up and down gently three times to mix.6. Continue diluting samples in the 8-tube strip.Remove 5 µl of sample from tube 3 and add it to tube 4. Mix gently.• Remove 5 ul of sample from tube 4 and add it to tube 5. Mix gently.Remove 5 ul of sample from tube 5 and add it to tube 6. Mix gently.7. Remove 5 µl of sample from tube 6 and discard.Tubes 1-6 should all have 5 µl of sample in each tube.• Tubes 1-6 now have a 2-fold serial dilution series.• Tube 7 should have no DNA and will serve as a blank control.8. Add 5 µl of the "Unknown DNA Concentration" to tube U.• The teacher will have "Unknown DNA Concentration" at the front of the room.9. Add 10 µl of Dye to all 8 tubes.10. Add 10 µl of Buffer to all 8 tubes.11. Cap the 8-strip of PCR tubes and place in P51TM or other blue light illuminator.• Darken the room, or use a light blocking hood to better view the samples.12. Estimate the concentration of DNA in the unknown from your dilution series.Concentration of 50:50 AT:GC DNA before adding to tube 1 was 1.5 µM (micromolar).• Brightness of the unknown sample is likely to not match any one tube in the dilution seriesexactly. Use a best approximation.

The results of a polymerase chain reaction (PCR) are significantly influenced by the overall amount…

2. To tube 1, add 10 μl of 50:50 AT:GC DNA (tube B). 3. Add 5 μl of Buffer to tubes 2-7. 4. Remove 5 μl from tube 1 and add it to tube 2. • Pipette up and down gently three times to mix. 5. Remove 5 μl from tube 2 and add it to tube 3. • Pipette up and down gently three times to mix. 6. Continue diluting samples in the 8-tube strip. • Remove 5 μl of sample from tube 3 and add it to tube 4. Mix gently.

2. To tube 1, add 10 μl of 50:50 AT:GC DNA (tube B). 3. Add 5 μl of Buffer to tubes 2-7. 4. Remove 5 μl from tube 1 and add it to tube 2. • Pipette up and down gently three times to mix. 5. Remove 5 μl from tube 2 and add it to tube 3. • Pipette up and down gently three times to mix. 6. Continue diluting samples in the 8-tube strip. • Remove 5 μl of sample from tube 3 and add it to tube 4. Mix gently.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter14: Biotechnology And Society

Section: Chapter Questions

Problem 9QP

See similar textbooks

Similar questions

DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to each of the experimental tubes. What would happen if you underloaded the enzyme? i.e. you only delivered 3 or 4 μl ? What would see in your gel results?
DUE IN 10 MINS. Answer only What is the enzyme that eventually joins the sugar-phosphate backbones of the Okazaki fragments and binds the DNA in DNA recombination? a. helicase b. restriction enzymes c. ligase d. exonuclease
Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In simple terms so, that I can understand. Thank you
Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?
Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)
Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)
Which of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
Answer the following questions. 1. Explain the steps associated with DNA profiling and state its advantages and disadvantages, 2. Describe how mutations are linked to DNA polymorphism. GUIDELINES: Each answer should be 250 words in length. Content should not be copied. References and bibliography should be provided for the content and images. please asap
1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total volume into an agarose gel. What was the original concentration of the loading dye? a. 4x b. 5x c. 6x d. 7x e. None of the above 1. b)A technologist adds 6uL of 5x loading dye to a DNA sample before adding the total volume into the agarose gel. How much DNA sample was combined with the loading dye? 12uL 16uL 20uL 24uL 28uL
show the findings (DNA extraction-gel band; NO/YES) and briefly explain the interpretation of the results
options for each are: primase, topoisomerase, dnaB, dnaA, Pol III
13. Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes Group of answer choices Primer Walking Positional Cloning Directional Cloning Chromosome Walking