2. Why is there a need to have a replicate for the experiment? 1. Which title best describes the research paper?O A. Effects of Substance A and B to the growth of OnionsO B. Effects of Substance A and B to the cell division of onion cells as manifested in the growth of roots and shootsO C. The growth of Onion root and shoot as affected by Substance A and BO D. Growing Onion Bulbs using Water, Substance A and Substance B as a substrate and nutrient source2. Why is there a need to have a replicate for the experiment?O A. To offer an elaborate methodology and make research more challengingO B. To create a more extensive procedure to make the research look consistentO C. To provide variety of result and data in case other replicates failO D. To have more data used in the statistical analysis Title:Research Using Scientific MethodDescription:A. Research Using the Scientific MethodMany researches had made use of the scientific method to device a scientific query that will test the effect of different substances to the cell division by observing changes inthe organisms' physical attributes. In this activity, two substances, A and B, are tested on their effects on the cell division of onion plant. Onion bulbs were submerged andallowed to grow together with the test substances and the length and numberexperiment. Use the table and analyze before you answer the questions that follow.Here is a video that shows the procedure of growing onions using water. The experiment is based on this. Use only as a reference. https://www.youtube.com/watch?v=QroXnfmhVTggrowing roots and shoots were measured. A table is provided showing the results of theLength of roots (inmm)Number of root Length ofgreen leaf (inmm)Number ofgrowthsprouted green leafDistilledReplicate 1432waterReplicate 24.2Replicate 36.442Substance Replicate 1AReplicate 262Replicate 3633Substance Replicate 1212621Replicate 2Replicate 33232

Replicates are copies of the sample that is being tested. These replicates are used in the…

2. Why is there a need to have a replicate for the experiment? O A. To offer an elaborate methodology and make research more challenging O B. To create a more extensive procedure to make the research look consistent O C. To provide variety of result and data in case other replicates fail O D. To have more data used in the statistical analysis

2. Why is there a need to have a replicate for the experiment? O A. To offer an elaborate methodology and make research more challenging O B. To create a more extensive procedure to make the research look consistent O C. To provide variety of result and data in case other replicates fail O D. To have more data used in the statistical analysis

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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1. Write if the statement is true and modify the statement with the correct answer if its false a. E. coli strains used in blue-white screening produce blue color complexes because their beta-galactosidases are activated via alpha-complementation. b. If you are a researcher trying to identify a gene that is responsible for a trait of interest, the first thing that you would do is to make a review of the literature that is available. c. The polylinker region in plasmids is where specific DNA sequences are cut by non-specific restriction enzymes. d. Blue-white screening is one of the last steps involved in determining if the gene of interest has been inserted successfully in plasmids.
1. Write if the statement is true and modify the statement with the correct answer if its false a.White colonies represent those bacteria that do not express the genes for beta-galactosidase because restriction enzymes have cut the gene and ligase have not repaired the break b.Antibiotic resistance genes in bacterial transformation are used as marker genes because of ease of detection of transformants. c. Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell.
1. It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector.A. Cosmid B. Plasmid. C. Phagemid. D. Genome 2. In plant genetic engineering, which of the following acts as vector?A. Agrobacterium lumefaciens. B. Gene of interest C Receipt plant cell. D. Ti-plasmid 3. Which of the following illustrates breeding?A. A farmer choose a breed of cow for greater milk productionB. The use of bacteria in order to produce human insulin.C. The insertion of cloned genes to plant cells.D. All of the above. 4. Being pest resistance is one of the traits that are being introduced to plants like corn and eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is inserted to plants?A. Biolistic B. Electroporation C. Plasmid insertion by heat shock treatment D. All of these 5. A biotechnologist wants to enhance the size of duhat fruit because research shows that it can lower blood sugar but is very…
7. Why recombinant DNA is very useful in improving our health conditions?A. Human insulin can be reproduced by bacteria?B. Vaccine can be reproduced by fungi or bacteriaC. Human growth hormone can be obtained from E. ColiD. All of the above 8. Which of the following is/are example of genetic engineering?A. A farmer choose a breed of cow for greater milk production.B. The use of bacteria in order to produce human insulinC. The insertion of clone genes to plant cellsD. Cross pollination of squash flowers. 9. Bacterial cells as host organisms may expose the gene to make protein products such as insulin and growth hormone . Which of the following processes are involved?A. replication and transcription. B. Transcription and replication C. Transcription and transformation. D. Replication and transformation
1. Write if the statement is true and modify the statement with the correct answer if its false a.Blue colonies in the white-blue screening provide sufficient information on the transfer of recombinant DNA in bacterial cells. b.In blue-white screening, it is only the blue colonies that have survived the effect of antibiotics in the media. c.Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell. d.Competent cells are those bacterial cells that can accept the entry or re-entry of plasmids.
1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis 2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.
1. Propose a way that a chi square analysis could be used in other experiments, such as genetics ordrug trials.
1. Illustrate the steps in restriction digestion and PCR 2.Discuss how PCR may be used for the detection of disease-causing pathogens in a population during the COVID Pandemic. For example: it may be used to check if a patient has a COVID virus infection. 3. Discuss how the cloning and expression of certain genes allows for massive production of the desired product. For Example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.
38. Stems cells can be used in the following medical technologies. A. gene therapy B. stem cell therapy C. 3D bioprinting as a bio-ink D.all of these E. none of these
1. What do we need to consider in choosing the best method in introducing recombinant DNA? Compare and contrast at least three considerations (in terms of methods or requirements) in introducing recombinant DNA in plant calli vs animal cell lines. Answer this comprehensively and clearly.
Primary cultures and limited cell lines have a low plating efficiency, which will influence cell survival rates. Make suggestions about how to increase the cloning procedure's plating efficiency.
1-What are the requirements to carry PCR? 2-How amplification will be specific for each step