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- How does the spectrophotometer measure absorbance? Why does the dye absorb light (electron transitions)? What color light does Blue #1 dye absorb? What is the lambda max of the dye? What is Beer’s law and how can it be used to calculate the desired concentrations for your solutions? Considering Beer’s law, is it possible to look up the molar absorptivity coefficient of Blue #1 dye? How will you estimate what concentrations of dye will be required for an absorbance of 1.0 AU given a path length of 1.46cm? How would you dilute a 2.0 mM dye solution to make 100mL of this 1.0 AU solution? How can the 1.0 AU solution be diluted to form a point on the calibration curve at 0.25 AU?Part a. Regarding micelles, do the electrostatic interactions between the sulfate head groups favor or destabilize micelle formation? How would dielectric interactions very in water vs ethanol if the dielectric constant of ethanol is 24.5 at room temperature. Part b. Why is the critical micelle concentration lower in a salt solution in terms of the Gibbs free energy?Please explain to me what is the valency factor (n-factor) of Potassium Bipthalate (C8H5KO4)
- Calculate the Debye length (1/k) of the double electric layer near the charged surface in KCl solutions of two concentrations c1 =0.01 M and c2= 0.1 M. Assume T= 300K, e = 80, k = 1.38 ·10-23 J/K, eo = 8.85 · 10-12 CV-1m-1 Tip: express concentrations as numbers of particles per liter (molarity×NA/L)Ksp for Co(OH)2 is 5.92x10^-15What is “the hydrophobic effect” and why it is so important to the molecular recognition and supramolecular chemistry?
- Construct the expression for Ksp for solid Ba₃(PO₄)₂ in aqueous solution. Ba₃(PO₄)₂(s) ⇌ 3 Ba²⁺(aq) + 2 PO₄³⁻(aq)Which is correct for polarography? I-polarography is an amperometry method conducted with a dripping Hg electrode. II-The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III-The half-wave potential is characteristic of the analyte in a given environment and can be used for qualitative analysis. IV-fresh Hg drops provide reproducible results. V-polarography is primarily used to study oxidations, Hg can be reduced when studying reductionsBeer's law why does the procedure for measuring the concentration of a solution photometrically require the use of a "blank"?
- Help me.... Explain the principles of gel permeation chromatography in Polymer, what is the application in Polymer analyticsAn experiment was carried out to determine desorption efficiency. I placed25 µL of hydrochloric acid solution (4350 ppm) onto 100 mg of silica gel.The next day I desorbed the HCl with 10 mL of standard eluent. 5 mL ofthis solution was found to contain 42 µg of hydrogen chloride. What is thedesorption efficiency?Calculate the osmolarity (mOsm/L) of D51/2NS (dextrose 5%/0.45% NaCl). Round your answer to the nearest ten. NaCl: MW 58.5, # particles on dissociation 2 Dextrose: MW 180, # particles on dissociation 1