Which is correct for polarography? I-polarography is an amperometry method conducted with a dripping Hg electrode. II-The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III-The half-wave potential is characteristic of the analyte in a given environment and can be used for qualitative analysis. IV-fresh Hg drops provide reproducible results. V-polarography is primarily used to study oxidations, Hg can be reduced when studying reductions

Which is correct for polarography? I-polarography is an amperometry method conducted with a dripping Hg electrode. II-The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III-The half-wave potential is characteristic of the analyte in a given environment and can be used for qualitative analysis. IV-fresh Hg drops provide reproducible results. V-polarography is primarily used to study oxidations, Hg can be reduced when studying reductions

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter25: Voltammetry

Section: Chapter Questions

Problem 25.11QAP

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Which are true for polarography? I Polarography is amperometry conducted with a dropping Hg electrode. II The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III The half-wave potential for maximum current is characteristic of a given analyte in a given medium and can be used for qualitative analysis for the analyte. IV Fresh Hg drops give reproducible results. V Polarography is used to primarily to study oxidations Hg may reduce when studying reductions. A.I, III, and IV B. II, III, and V C. I and V D. III, IV, and V E. II, III, and IV
The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards on an SDS– polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass of a protein having a mobility of 0.62 on this gel?
what is Optical rotatory dispersion and circulatory dichroism method for protein binding?
One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained. ppm, P aBSORBANCE (A) 1.003.00 0.230 2.00 0.436 3.00 0.638 4.00 0.848 Urine sample 0.518 ffind the slope and intercept, then graph it.
2 d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the column. Why is high pressure needed in HPLC? e) Why are HPLC particles porous?
Proteins A, B, and C have isoelctric points of 8.2, 6.7, and 5.2 respectively. Place proteins A, B, and C in order of increasing volume on a size exclusion column. Then place the proteins in order of probable increasing elution volume in cation exchange chromatography during an increasing salt graditend run at pH 5.
The soxhlet extraction method was applied to a solid food sample that matrix effects are expected to be significant for the total lipid analysis. The sample was directly subjective for extraction, and ethanol was used as the extraction solvent. (a.) Outline the typical procedure of the Soxhlet extraction method (b.) Calculate the per cent lipid of the food sample if the result is as following: -Food sample: 12.5755 g -Initial weight of the solvent flask: 30.5697 g -Weight of the solvent flask treated with the ethanol: 30.6271 g -Weight of the solvent flask treated with the sample: 34.0293 g (c.) The value of total lipid content obtained from (b) was much lower than that expected. Suggest three possible reasons and the respective modification improve the analysis.
Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric points 4,3, 8,7 and 9,2 is applied to a column containing an anion-exchanging filler.
Which statements are true for molecular exclusion chromatography?I Molecular exclusion chromatography separates on the basis of size.II Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume, and elute last.III The pores in the stationary phase are too small for large molecules to pass through, so large molecules elute first.IV Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography. A. I, II, III B. III and IV C. II only D. I and II only E. I, III, and IV
The separation of APAP-L,(12 = 0.28 min) from APAP-D (W12 = 0.35 min) on a chiral stationary phase was found to have retention times of 4.78 min and 5.26 min, respectively. With a unretained solute being, found to have a retention time of 1.1 min on the same column. Calculate the column resolution and selectivity factor
The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards on an SDS-polyacrylaide gel are 0.80 and 0.41. What is the apparent mass of a protein having a mobility of 0.62 on ths gel?
A measurment for the Lambda-max for a colored solution is 671 nm with a very high Absorptivity. What does the color of the solution look like in the sunshine? and What does the color look like in a room where the only light source emits 671 nm