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250 uL of 1M azide was added to a 25 mL yeast solution.
What was the concentration of azide in the yeast culture? (use proper units)
- Azide is a strong inhibitor of yeast mitochondrial respiration.
- It obstructs the electron transport chain, preventing ATP production.
- Azide is frequently used in laboratories to investigate the effects of inhibiting this process on cellular metabolism.
- It can also be used as a preservative in yeast cultures to inhibit bacterial development.
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- in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g Saccharomyces cerevisiae to 3 mL yeast growing medium (56 mM glucose, 20 mM HEPES, pH 6.8). What percent of yeast suspension is left after a 1:10 dilution?Was there any difference in the amount of gas produced by yeast in room temperature tapwater compared with yeast in tapwater at 35-40 degrees Centigrade? Explain why this might be so.Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.
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