in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g Saccharomyces cerevisiae to 3 mL yeast growing medium (56 mM glucose, 20 mM HEPES, pH 6.8). What percent of yeast suspension is left after a 1:10 dilution?

in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g Saccharomyces cerevisiae to 3 mL yeast growing medium (56 mM glucose, 20 mM HEPES, pH 6.8). What percent of yeast suspension is left after a 1:10 dilution?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 10SST

See similar textbooks

Related questions

Q: Explain in molecular terms how a bacterial cell is made lysogenic by a temperate phage such a lambda

A: Introduction :- Lysogeny is the process in which a temperate phage, such as lambda, infects a…

Q: CO-DOMINANCE/I... 2/3 | All 1 87% + 3. A homozygous red gummy bear is crossed with a homozygous…

A: Introduction :- A Punnett Square is a tool used to predict the offspring of a genetic cross between…

Q: draw a punnett square for ggBB and GGBb.

A: The genotypes of an individual refer to the arrangement or the type of gene combination that an…

Q: Earlobes can be attached to the face or non-attached. An attached earlobe is a recessive trait. A…

A: Mother is having attached earlobes with a genotype of ee. Father is having non-attached earlobes and…

Q: Suppose that allele D produces tiny ears and allele d produces normal ears. A heterozygous male with…

Q: Briefly describe envelop virus enzymes and proteins.*keep it short*

A: Viruses are tiny infectious agents that can replicate only inside the cells of other organisms. They…

Q: Discuss many recent techniques for enhancement and restoration.

A: ANSWER: Recent techniques for image enhancement and repair include: Superior Machine Learning:…

Q: Heparin is a blood-thinning drug (prevents coagulation). Heparin is broken down by the liver, and…

A: The term "half-life" describes how long it takes for a substance, such as a drug, to be completely…

Q: The centromeric connections between chromatids fail to be severed. Kinetochores from sister…

A: Chromosomes' centromeres are narrow regions that hold two sister chromatids together. Additionally,…

Q: provide study and literature about aquaponics use google scholar for articles questions: 1. why…

A: Aquaponics is a method of farming where fish and plants are grown together in a closed-loop system.…

Q: The small figures at the bottom of the figure labeled a-f represent key events in primate evolution…

A: Evolution of steady phenomenon which brings about alteration in life from much simple to more…

Q: Iron atoms have been detected in the sun's atmosphere, some with many of their electrons stripped…

A: Atoms are made up of fundamental particles called electrons and protons. Protons have a positive…

Q: Please explain the process of DNA Replication.

A: INTRODUCTION The process of duplicating the genetic data included in a cell's DNA is known as DNA…

Q: strains of Drosophila that have either normal legs or abnormally short legs and you are studying the…

A: Law of dominance says that dominant character is expressed in two conditions either in homozygous…

Q: Describe the anatomical and directional terms in locating the digestive structures of toad, fish and…

A: Introduction :- The digestive system is a series of organs that work together to break down food,…

Q: Xeriscaping Research

Q: can someone explain these calculations to me?

A: At first, a standard curve is made using the OD of protein of known concentrations, i.e., ODs…

Q: In a Gecko population a group of Geckos skin color is controlled by one gene with two alleles…

A: The chi-square (χ²) statistic is used to detect whether or not there is a statistically significant…

Q: Differentiate the activity of an RNA-dependent RNA polymerase when functioning as a replicase and…

A: Introduction :- In transcription, the DNA double helix unwinds and one strand acts as a template for…

Q: Do dinoflagellates push or pull themselves through the water with their flagella? How does this…

A: Introduction In 5 kingdom classification, there are 5 kingdoms include monera, protista, fungi,…

Q: The Siamese cat breed has a light‑colored body and dark‑colored head, tail, and feet, all of which…

A: Cross breeding, also known as hybridization, is the process of breeding two different species or…

Q: Provide the major organelles involved in protein synthesis and packaging.

A: Protein synthesis involves four different organelles. These comprise the ribosomes, rough…

Q: Parents purple x purple red x blue white x white red x white precursor 1 precursor 2 he pathway…

A: Enzymes are responsible for a certain pathway to be carried on. In this various precursors are…

Q: Flowering plants are greatly different from conifers and other gymnosperms in their: O Parenchyma,…

A: INTRODUCTION Flowering Plants Flowering plants, also known as angiosperms, are plants that produce…

Q: Explain how aging and lifestyle can affect the health and strength of bone.

A: Our skeleton system is made up of bones. There are 206 bones present in adult human. According to…

Q: 3. What health teachings can you give to the donor of the fecal sample who was found to have these…

A: The donor of a fecal sample has been found to have parasites, which can cause a range of health…

Q: The difference between continuous and discontinuous variation.

A: In biology, variations are differences between two living organisms or groups of living organisms.…

Q: in meiosis, does the DNA duplicate

A: Introduction :- Meiosis is a type of cell division that reduces the number of chromosomes in a cell…

Q: A scientist placed a sample of yeast, a heterotrophic single-celled eukaryote, in a closed test tube…

A: Introduction :- Yeast is a type of single-celled fungus that belongs to the Kingdom Fungi. It is a…

Q: Search Chemical Fa

A: Introduction: A chemical formula is a concise representation of a chemical substance that shows its…

Q: At Elmhurst hospital in Queens, a couple originally from the South Indian city of Bengaluru bring…

A: The situation in the waiting room of Elmhurst Hospital in Queens is tense. A couple originally from…

Q: Which form of cooking will result in meat having heme (Fe) groups being bound to nitric oxide?…

A: Food processing involves series of steps by employing various techniques to preserve food as well…

Q: mitosis nuclei resides in the nucleus karyotype autosomes haploid In dividing parent cell; in the…

A: Introduction :- Meiosis is a type of cell division that results in the formation of genetically…

Q: If no parasite was detected in the samples, what could be the possible reason/s. What is the…

A: Note:- sorry The firdt part of the question cannot be answered as it seems to be missing some…

Q: Stru

A: Introduction: Bones are formed of a variety of cells, proteins, minerals, and vitamins and are…

Q: 2. How can parasites (helminths & protozoa) be possibly spread from an infected person to healthy…

A: The term "parasite" refers to an organism that depends on its "host" in order to survive. Some…

Q: role of iron (Fe) in human body by focusing on Structure-Function-Location • how much of FE is…

A: Iron is an example of a micronutrient mineral that is needed in very small amounts by humans for a…

Q: provide a paragraph on sexual dimorphism. discuss why some species have pronounced dimorphism, and…

A: Introduction :- Sexual dimorphism refers to the differences in physical appearance and…

Q: What recommendations would you make to Cass to help her maintain healthy blood sugar levels? What…

A: It is very necessary to maintain healthy blood sugar level. This prevents or delay long-term,…

Q: What is gluconeogenesis? 2. Explain the biochemical effects underlying the metabolic functions of…

A: Gluconeogenesis is very much related to the synthesis of glucose. The level of glucose is very much…

Q: 29. Observe this chemical reaction to answer the following questions (29a through 29h). "Reaction…

A: Note: Since you have posted a question with multiple sub-parts, we will solve the first three…

Q: My options are 0.287 0.122 0.234 0.367

A: In genetics, dominant and recessive refer to the relationship between two versions of a gene, known…

Q: 5. The electrochemical gradient can be utilised by ATP synthase to generate ATP - explain how the…

A: Photosynthesis - It is a process in which energy from sunlight is transformed into chemical energy…

Q: there a correlation between survival, reproduction and environment color? Explain. 2. Are there any…

A: Natural selection is the major reason to assess the survival rate. Natural selection is the concept…

Q: Passive nonvoluntary euthanasia is the one kind of euthanasia that is almost always morally…

A: Euthanasia is the act of intentionally ending the life of a person in order to relieve them of…

Q: Discuss the impact of the Mandatory requirement of CPD in the practice of medical technology.

A: CPD stands for continuing professional development. It includes programs that help medical…

Q: What structures or features do all protists have in common?

A: All eukaryotes that are not fungi, animals, or plants are categorised as protozoa, or protists.…

Q: (5)Baldness is an X-recessive linked trait. Baldness is epistatic to curly hair" which is dominant…

A: Baldness is X-linked and recessive, which means that any male with an X chromosome with a baldness…

Q: One main purpose for hemicelluloses in the cell-wall structure is to act as a coupling agent between…

A: Introduction: The second most prevalent biopolymer after cellulose is hemicellulose, which is…

Q: key points from attached

A: The study was conducted in a population where pre and post covid pandemic sexual behaviors were…

Concept explainers

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

Question

in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g Saccharomyces cerevisiae to 3 mL yeast growing medium (56 mM glucose, 20 mM HEPES, pH 6.8).

What percent of yeast suspension is left after a 1:10 dilution?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

given a phage lysate with 10^11 pfu/ml and a bacterial culture with 10^8 cfu/ml, how would you obtain MOI =0.1 ?
What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include source
Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?
Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.
How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plate
How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM colchione?
Why do boiled and unboiled potato extracts differ in their action on hydrogen peroxide? Explain in 1-3 sentences
Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen storage in crypto preservation? In addition, why are antibiotics not used in the cell freezing media? Why FBS concentration higher than 10% (10% is the standard concentration in complete growth media) is recommended in cell freezing media?
How much nystatin stock (50 mg/mL) needs to be added to 15 mL of tryptic soy agar to give a final concentration of 50 μg/mL?
. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…
Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic acid Indole glycerol phosphate Indole Tryptophan trp-1 − − − − + trp-2 − − + + + trp-3 − − − + + trp-4 − − + + + trp-6 − − − − + trp-7 − − − − + trp-8 − + − − + trp-9 − − − − + trp-10 − − − − + trp-11 − − − − + Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations.