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- Which stationary phase is better in separation of the components of moringa extract for column chromatography? Sodium Bicarbonate or Silica Gel? And Explain.What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?How to calculate for FAME yield given the dried FAME extracted of 0.021 g and the volume of culture used is 10 mL?
- How much nystatin stock (50 mg/mL) needs to be added to 15 mL of tryptic soy agar to give a final concentration of 50 μg/mL?What types of macromolecules are usually separated on agarose electrophoresis gels?How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?
- A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?What is meant by the term plating efficiency?How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
- In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?What is the basic purpose of the paper chromatography?You have to prepare an agarose solution for gel electrophoresis. How many grams of agarose powder do you need in order for you to make 50mL of 1% agarose solution?